3okg

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Current revision (11:17, 21 February 2024) (edit) (undo)
 
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<StructureSection load='3okg' size='340' side='right'caption='[[3okg]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='3okg' size='340' side='right'caption='[[3okg]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3okg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cals4 Cals4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OKG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3okg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OKG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3okg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3okg OCA], [https://pdbe.org/3okg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3okg RCSB], [https://www.ebi.ac.uk/pdbsum/3okg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3okg ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3okg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3okg OCA], [https://pdbe.org/3okg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3okg RCSB], [https://www.ebi.ac.uk/pdbsum/3okg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3okg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8R9Q6_CALS4 Q8R9Q6_CALS4]
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Type I DNA methyltransferases contain one specificity subunit (HsdS) and two modification subunits (HsdM). The electron microscopy model of M.EcoKI-MS methyltransferase shows a reasonable closed state of this clamp-like enzyme, but the structure of the open state is still unclear. The 1.95 A crystal structure of the specificity subunit from Thermoanaerobacter tengcongensis (TTE-HsdS) shows an unreported open form inter-domain orientation of this subunit. Based on the crystal structure of TTE-HsdS and the closed state model of M.EcoKI-MS, we constructed a potential open state model of type I methyltransferase. Mutational studies indicated that two alpha-helices (aa30-59 and aa466-495) of the TTE-HsdM subunit are important inter-subunit interaction sites in the TTE-MS complex. DNA binding assays also highlighted the importance of the C-terminal region of TTE-HsdM for DNA binding by the TTE-MS complex. On the basis of structural analysis, biochemical experiments and previous studies, we propose a dynamic opening and closing mechanism for type I methyltransferase.
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Structure of HsdS subunit from Thermoanaerobacter tengcongensis sheds lights on mechanism of dynamic opening and closing of type I methyltransferase.,Gao P, Tang Q, An X, Yan X, Liang D PLoS One. 2011 Mar 2;6(3):e17346. PMID:21399684<ref>PMID:21399684</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3okg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cals4]]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: An, X]]
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[[Category: An X]]
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[[Category: Gao, P]]
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[[Category: Gao P]]
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[[Category: Liang, D]]
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[[Category: Liang D]]
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[[Category: Tang, Q]]
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[[Category: Tang Q]]
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[[Category: Yan, X]]
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[[Category: Yan X]]
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[[Category: Coiled-coil]]
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[[Category: Dna binding]]
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[[Category: Dna binding protein]]
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[[Category: Type i methyltransferase]]
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Current revision

Crystal structure of HsdS subunit from Thermoanaerobacter tengcongensis

PDB ID 3okg

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OCA

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