3oth

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<StructureSection load='3oth' size='340' side='right'caption='[[3oth]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='3oth' size='340' side='right'caption='[[3oth]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3oth]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.301&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLJ:CALICHEAMICIN+ALPHA3I'>CLJ</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3otg|3otg]], [[3oti|3oti]], [[3d0r|3d0r]], [[3d0q|3d0q]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oth OCA], [https://pdbe.org/3oth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oth RCSB], [https://www.ebi.ac.uk/pdbsum/3oth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oth ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8KNF2_MICEC Q8KNF2_MICEC]
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Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
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Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3oth" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 15837]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Bingman, C A]]
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[[Category: Micromonospora echinospora]]
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[[Category: Structural genomic]]
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[[Category: Bingman CA]]
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[[Category: Chang, A]]
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[[Category: Chang A]]
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[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
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[[Category: Phillips Jr GN]]
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[[Category: Phillips, G N]]
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[[Category: Singh S]]
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[[Category: Singh, S]]
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[[Category: Thorson JS]]
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[[Category: Thorson, J S]]
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[[Category: Calicheamicin]]
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[[Category: Cesg]]
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[[Category: Enzyme discovery for natural product biosynthesis]]
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[[Category: Glycosyltransferase]]
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[[Category: Gt-b fold]]
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[[Category: Natpro]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Tdp]]
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[[Category: Transferase-antibiotic complex]]
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Revision as of 11:18, 21 February 2024

Crystal Structure of CalG1, Calicheamicin Glycostyltransferase, TDP and calicheamicin alpha3I bound form

PDB ID 3oth

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