1qk7
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1qk7.jpg|left|200px]] | [[Image:1qk7.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1qk7", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1qk7| PDB=1qk7 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR''' | '''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR''' | ||
| Line 28: | Line 25: | ||
[[Category: Liang, S.]] | [[Category: Liang, S.]] | ||
[[Category: Lu, S.]] | [[Category: Lu, S.]] | ||
| - | [[Category: | + | [[Category: Cystine knot]] |
| - | [[Category: | + | [[Category: Lectin]] |
| - | [[Category: | + | [[Category: Selenocosmia huwena]] |
| - | [[Category: | + | [[Category: Shl-i]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:22:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:22, 3 May 2008
SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR
Overview
The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
About this Structure
1QK7 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR., Lu S, Liang S, Gu X, J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779 Page seeded by OCA on Sat May 3 06:22:21 2008
