This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1qkg
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1qkg.gif|left|200px]] | [[Image:1qkg.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1qkg", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1qkg| PDB=1qkg | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT''' | '''DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKG OCA]. | |
==Reference== | ==Reference== | ||
The Dewar photoproduct of thymidylyl(3'-->5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10758155 10758155] | The Dewar photoproduct of thymidylyl(3'-->5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10758155 10758155] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Bae, S H.]] | [[Category: Bae, S H.]] | ||
[[Category: Choi, B S.]] | [[Category: Choi, B S.]] | ||
[[Category: Choi, Y J.]] | [[Category: Choi, Y J.]] | ||
[[Category: Lee, J H.]] | [[Category: Lee, J H.]] | ||
| - | [[Category: | + | [[Category: Dewar product]] |
| - | + | [[Category: Dna photoproduct]] | |
| - | [[Category: | + | [[Category: Mutagenesis]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | [[Category: Translesion replication]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:22:56 2008'' |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:22, 3 May 2008
DNA DECAMER DUPLEX CONTAINING T-T DEWAR PHOTOPRODUCT
Overview
In contrast to the highly mutagenic pyrimidine(6-4)pyrimidone photoproduct, its Dewar valence isomer (Dewar product) has low mutagenic potential and produces a broad range of mutations [LeClerc, J. E., Borden, A. & Lawrence, C. W. (1991) Proc. Natl. Acad. Sci. USA 88, 9685-9689]. To determine the origin of the mutagenic property of the Dewar product, we used experimental NMR restraints and molecular dynamics to determine the solution structure of a Dewar-lesion DNA decamer duplex. This DNA decamer duplex (DW/GA duplex) contains a mismatched base pair between the 3' T residue of the Dewar lesion (T6) and an opposed G residue (G15). The 3' T (T6) of the Dewar lesion formed stable hydrogen bonds with the opposing G15 residue. However, the helical bending and unwinding angles of the DW/GA duplex were much larger than those of a second duplex that contains the Dewar lesion and opposing A15 and A16 residues (DW/AA duplex). The DW/GA duplex showed poorer stacking interactions at the two bases of the Dewar product and at the adjacent A7 small middle dotT14 base pair than did the DW/AA duplex. These structural features imply that no thermal stability or conformational benefit is obtained by incorporating a G instead of an A opposite the 3' T of the Dewar lesion. These properties may thus facilitate the preferential incorporation of an A in accordance with the A rule during translesion replication and lead to the low frequency of 3' T-->C mutations observed at this site.
About this Structure
Full crystallographic information is available from OCA.
Reference
The Dewar photoproduct of thymidylyl(3'-->5')- thymidine (Dewar product) exhibits mutagenic behavior in accordance with the "A rule"., Lee JH, Bae SH, Choi BS, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4591-6. PMID:10758155 Page seeded by OCA on Sat May 3 06:22:56 2008
