3rv1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:45, 1 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='3rv1' size='340' side='right'caption='[[3rv1]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
<StructureSection load='3rv1' size='340' side='right'caption='[[3rv1]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3rv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_polysporus Kluyveromyces polysporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RV1 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3rv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora_DSM_70294 Vanderwaltozyma polyspora DSM 70294]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RV1 FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rv0|3rv0]]</div></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.975&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpol_455p11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436907 Kluyveromyces polysporus])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv1 OCA], [https://pdbe.org/3rv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rv1 RCSB], [https://www.ebi.ac.uk/pdbsum/3rv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rv1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv1 OCA], [https://pdbe.org/3rv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rv1 RCSB], [https://www.ebi.ac.uk/pdbsum/3rv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rv1 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A7TR32_VANPO A7TR32_VANPO]
-
The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) that direct RNA interference. Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeasts. The crystal structure revealed a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, and it identified additional catalytic residues conserved throughout eukaryotic RNase III enzymes. Biochemical analyses showed that Dcr1 dimers bind cooperatively along the dsRNA substrate such that the distance between consecutive active sites determines the length of the siRNA products. Thus, unlike canonical Dicers, which successively remove siRNA duplexes from the dsRNA termini, budding-yeast Dicers initiate processing in the interior and work outward. The distinct mechanism of budding-yeast Dicers establishes a paradigm for natural molecular rulers and imparts substrate preferences with ramifications for biological function.
+
-
 
+
-
The inside-out mechanism of dicers from budding yeasts.,Weinberg DE, Nakanishi K, Patel DJ, Bartel DP Cell. 2011 Jul 22;146(2):262-76. PMID:21784247<ref>PMID:21784247</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3rv1" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Kluyveromyces polysporus]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Bartel, D P]]
+
[[Category: Vanderwaltozyma polyspora DSM 70294]]
-
[[Category: Nakanishi, K]]
+
[[Category: Bartel DP]]
-
[[Category: Patel, D J]]
+
[[Category: Nakanishi K]]
-
[[Category: Weinberg, D E]]
+
[[Category: Patel DJ]]
-
[[Category: Rna binding protein]]
+
[[Category: Weinberg DE]]
-
[[Category: Rnase iii enzyme]]
+

Current revision

Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant

PDB ID 3rv1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rv1"
Personal tools