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3rwl

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Current revision (09:45, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3rwl' size='340' side='right'caption='[[3rwl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3rwl' size='340' side='right'caption='[[3rwl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3rwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_51380 Atcc 51380]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RWL FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3rwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingopyxis_macrogoltabida Sphingopyxis macrogoltabida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RWL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ahpG1, Sphingomonas sp. ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33050 ATCC 51380])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkane_1-monooxygenase Alkane 1-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.3 1.14.15.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rwl OCA], [https://pdbe.org/3rwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rwl RCSB], [https://www.ebi.ac.uk/pdbsum/3rwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rwl ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rwl OCA], [https://pdbe.org/3rwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rwl RCSB], [https://www.ebi.ac.uk/pdbsum/3rwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rwl ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5F4D9_SPHMC Q5F4D9_SPHMC]
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Directed evolution of a monooxygenase to achieve very high enantioselectivity for hydroxylation at non-activated carbon atoms is demonstrated for the first time, where a triple mutant of P450pyr hydroxylase is obtained via determination of enzyme structure, iterative saturation mutagenesis, and high-throughput screening with a MS-based ee assay to increase the product ee from 53% to 98% for the hydroxylation of N-benzyl pyrrolidine to (S)-N-benzyl 3-hydroxypyrrolidine.
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Evolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atom.,Pham SQ, Pompidor G, Liu J, Li XD, Li Z Chem Commun (Camb). 2012 May 14;48(38):4618-20. Epub 2012 Mar 19. PMID:22430002<ref>PMID:22430002</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3rwl" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Alkane 1-monooxygenase]]
 
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[[Category: Atcc 51380]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Pompidor, G]]
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[[Category: Sphingopyxis macrogoltabida]]
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[[Category: Oxidoreductase]]
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[[Category: Pompidor G]]
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[[Category: P450 monooxygenase]]
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Current revision

Structure of P450pyr hydroxylase

PDB ID 3rwl

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