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3s4g

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Current revision (09:47, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3s4g' size='340' side='right'caption='[[3s4g]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
<StructureSection load='3s4g' size='340' side='right'caption='[[3s4g]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3s4g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Stnv1 Stnv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S4G FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3s4g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Satellite_tobacco_necrosis_virus_1 Satellite tobacco necrosis virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S4G FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rqv|3rqv]], [[1vtz|1vtz]]</div></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s4g OCA], [https://pdbe.org/3s4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s4g RCSB], [https://www.ebi.ac.uk/pdbsum/3s4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s4g ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s4g OCA], [https://pdbe.org/3s4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s4g RCSB], [https://www.ebi.ac.uk/pdbsum/3s4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s4g ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CAPSD_STNV1 CAPSD_STNV1] Self-assembles to form an icosahedral capsid of 17 nm in diameter.<ref>PMID:21839089</ref> <ref>PMID:23318955</ref> <ref>PMID:6481804</ref>
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A codon-optimised gene has been expressed in Escherichia coli to produce the coat protein (CP) of the Satellite Tobacco Necrosis Virus. This protein assembles in vivo into capsids closely resembling those of the T=1 wild-type virus. These virus-like particles (VLPs) package the recombinant mRNA transcript and can be disassembled and reassembled using different buffer conditions. The X-ray crystal structure of the VLP has been solved and refined at 1.4 A resolution and shown to be very similar to that of wild-type Satellite Tobacco Necrosis Virus, except that icosahedral symmetry constraints could be removed to reveal differences between subunits, presumably owing to crystal packing. An additional low-resolution X-ray crystal structure determination revealed well-ordered RNA fragments lodged near the inside surface of the capsid, close to basic clusters formed by the N-terminal helices that project into the interior of the particle. The RNA consists of multiple copies of a 3-bp helical stem, with a single unpaired base at the 3' end, and probably consists of a number of short stem-loops where the loop region is disordered. The arrangement of the RNA is different from that observed in other satellite viruses.
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Construction and Crystal Structure of Recombinant STNV Capsids.,Lane SW, Dennis CA, Lane CL, Trinh CH, Rizkallah PJ, Stockley PG, Phillips SE J Mol Biol. 2011 Oct 14;413(1):41-50. Epub 2011 Aug 3. PMID:21839089<ref>PMID:21839089</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3s4g" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Stnv1]]
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[[Category: Satellite tobacco necrosis virus 1]]
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[[Category: Dennis, C A]]
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[[Category: Dennis CA]]
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[[Category: Lane, C L]]
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[[Category: Lane CL]]
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[[Category: Lane, S W]]
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[[Category: Lane SW]]
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[[Category: Phillips, S E.V]]
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[[Category: Phillips SEV]]
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[[Category: Rizkallah, P J]]
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[[Category: Rizkallah PJ]]
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[[Category: Stockley, P G]]
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[[Category: Stockley PG]]
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[[Category: Trinh, C H]]
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[[Category: Trinh CH]]
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[[Category: Jelly-roll -sandwich]]
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[[Category: Low resolution structure]]
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[[Category: Protein-rna complex]]
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[[Category: Virus]]
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[[Category: Virus capsid protein]]
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Current revision

Low Resolution Structure of STNV complexed with RNA

PDB ID 3s4g

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