3sbo

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Current revision (09:49, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3sbo' size='340' side='right'caption='[[3sbo]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='3sbo' size='340' side='right'caption='[[3sbo]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3sbo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SBO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3sbo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH5alpha Escherichia coli DH5alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SBO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.204&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate_dehydrogenase_(NADP(+)) Glutamate dehydrogenase (NADP(+))], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.4 1.4.1.4] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbo OCA], [https://pdbe.org/3sbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sbo RCSB], [https://www.ebi.ac.uk/pdbsum/3sbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sbo ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sbo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbo OCA], [https://pdbe.org/3sbo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sbo RCSB], [https://www.ebi.ac.uk/pdbsum/3sbo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sbo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/DHE4_ECOLI DHE4_ECOLI]] Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.<ref>PMID:235298</ref> <ref>PMID:241744</ref>
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[https://www.uniprot.org/uniprot/DHE4_ECOLI DHE4_ECOLI] Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.<ref>PMID:235298</ref> <ref>PMID:241744</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structural biology and structural genomics projects routinely rely on recombinantly expressed proteins, but many proteins and complexes are difficult to obtain by this approach. We investigated native source proteins for high-throughput protein crystallography applications. The Escherichia coli proteome was fractionated, purified, crystallized, and structurally characterized. Macro-scale fermentation and fractionation were used to subdivide the soluble proteome into 408 unique fractions of which 295 fractions yielded crystals in microfluidic crystallization chips. Of the 295 crystals, 152 were selected for optimization, diffraction screening, and data collection. Twenty-three structures were determined, four of which were novel. This study demonstrates the utility of native source proteins for high-throughput crystallography.
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Macro-to-Micro Structural Proteomics: Native Source Proteins for High-Throughput Crystallization.,Totir M, Echols N, Nanao M, Gee CL, Moskaleva A, Gradia S, Iavarone AT, Berger JM, May AP, Zubieta C, Alber T PLoS One. 2012;7(2):e32498. Epub 2012 Feb 29. PMID:22393408<ref>PMID:22393408</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3sbo" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia coli]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Echols, N]]
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[[Category: Echols N]]
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[[Category: Gee, C L]]
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[[Category: Gee CL]]
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[[Category: Totir, M]]
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[[Category: Totir M]]
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[[Category: Zubieta, C]]
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[[Category: Zubieta C]]
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[[Category: Dehydrogenase]]
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[[Category: Oxidoreductase]]
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[[Category: Rossmann fold]]
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Current revision

Structure of E.coli GDH from native source

PDB ID 3sbo

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