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3sky

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Current revision (09:51, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3sky' size='340' side='right'caption='[[3sky]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='3sky' size='340' side='right'caption='[[3sky]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3sky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SKY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3sky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SKY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3skx|3skx]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AF_0152, copB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sky OCA], [https://pdbe.org/3sky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sky RCSB], [https://www.ebi.ac.uk/pdbsum/3sky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sky ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sky OCA], [https://pdbe.org/3sky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sky RCSB], [https://www.ebi.ac.uk/pdbsum/3sky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sky ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/COPB_ARCFU COPB_ARCFU]] Involved in copper export.<ref>PMID:12876283</ref> <ref>PMID:22663904</ref>
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[https://www.uniprot.org/uniprot/COPB_ARCFU COPB_ARCFU] Involved in copper export.<ref>PMID:12876283</ref> <ref>PMID:22663904</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Heavy metal P1B-type ATPases play a critical role in cell survival by maintaining appropriate intracellular metal concentrations. Archaeoglobus fulgidus CopB is a member of this family that transports Cu(II) from the cytoplasm to the exterior of the cell using ATP as energy source. CopB has a 264 amino acid ATP-binding domain (ATPBD) that is essential for ATP binding and hydrolysis as well as ultimately transducing the energy to the transmembrane metal binding site for metal occlusion and export. The relevant conformations of this domain during the different steps of the catalytic cycle are still under discussion. Through crystal structures of the apo- and phosphatebound ATPBDs, with limited proteolysis and fluorescence studies of the apo and substrate-bound states, we show that the isolated ATPBD of CopB cycles from an open conformation in the apo-state to a closed conformation in the substrate bound state, then returns to an open conformation suitable for product release. This work is the first structural report of an ATPBD with its physiologically-relevant product (phosphate) bound. The solution studies we have performed help resolve questions on the potential influences of crystal packing on domain conformation. These results explain how phosphate is coordinated in ATPase transporters, and give insight into the physiologically-relevant conformation of the ATPBD at different steps of the catalytic cycle.
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Conformations of the apo, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle.,Jayakanthan S, Roberts SA, Weichsel A, Arguello JM, McEvoy MM Biosci Rep. 2012 Jun 4. PMID:22663904<ref>PMID:22663904</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3sky" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arcfu]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Copper-exporting ATPase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Arguello, J M]]
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[[Category: Arguello JM]]
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[[Category: Jayakanthan, S]]
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[[Category: Jayakanthan S]]
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[[Category: McEvoy, M M]]
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[[Category: McEvoy MM]]
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[[Category: Roberts, S A]]
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[[Category: Roberts SA]]
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[[Category: Weichsel, A]]
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[[Category: Weichsel A]]
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[[Category: Amppnp]]
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[[Category: Atp binding domain]]
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[[Category: Hydrolase]]
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[[Category: Membrane protein]]
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[[Category: P1b-atpase]]
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[[Category: Phosphate ion]]
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Current revision

2.1A crystal structure of the phosphate bound ATP binding domain of Archaeoglobus fulgidus COPB

PDB ID 3sky

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