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| | <StructureSection load='3t97' size='340' side='right'caption='[[3t97]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='3t97' size='340' side='right'caption='[[3t97]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3t97]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T97 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3t97]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T97 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t98|3t98]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Nup54 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Nup62 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t97 OCA], [https://pdbe.org/3t97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t97 RCSB], [https://www.ebi.ac.uk/pdbsum/3t97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t97 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t97 OCA], [https://pdbe.org/3t97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t97 RCSB], [https://www.ebi.ac.uk/pdbsum/3t97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t97 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/NUP54_RAT NUP54_RAT]] Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. [[https://www.uniprot.org/uniprot/NUP62_RAT NUP62_RAT]] Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.
| + | [https://www.uniprot.org/uniprot/NUP54_RAT NUP54_RAT] Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The nuclear pore complex encloses a central channel for nucleocytoplasmic transport, which is thought to consist of three nucleoporins, Nup54, Nup58, and Nup62. However, the structure and composition of the channel are elusive. We determined the crystal structures of the interacting domains between these nucleoporins and pieced together the molecular architecture of the mammalian transport channel. Located in the channel midplane is a flexible Nup54Nup58 ring that can undergo large rearrangements yielding diameter changes from approximately 20 to approximately 40 nm. Nup62Nup54 triple helices project alternately up and down from either side of the midplane ring and form nucleoplasmic and cytoplasmic entries. The channel consists of as many as 224 copies of the three nucleoporins, amounting to a molar mass of 12.3 MDa and contributing 256 phenylalanine-glycine repeat regions. We propose that the occupancy of these repeat regions with transport receptors modulates ring diameter and transport activity.
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| - | | + | |
| - | Molecular architecture of the transport channel of the nuclear pore complex.,Solmaz SR, Chauhan R, Blobel G, Melcak I Cell. 2011 Oct 28;147(3):590-602. PMID:22036567<ref>PMID:22036567</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3t97" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Nucleoporin 3D structures|Nucleoporin 3D structures]] | | *[[Nucleoporin 3D structures|Nucleoporin 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blobel, G]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Chauhan, R]] | + | [[Category: Blobel G]] |
| - | [[Category: Melcak, I]] | + | [[Category: Chauhan R]] |
| - | [[Category: Alpha helical protein]] | + | [[Category: Melcak I]] |
| - | [[Category: Buried polar residue]]
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| - | [[Category: Central channel]]
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| - | [[Category: Central transport channel]]
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| - | [[Category: Coiled-coil]]
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| - | [[Category: Fg repeat]]
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| - | [[Category: Karyopherin]]
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| - | [[Category: Nuclear envelope]]
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| - | [[Category: Nuclear pore complex]]
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| - | [[Category: Nuclear pore membrane domain]]
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| - | [[Category: Nucleocytoplasmic transport]]
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| - | [[Category: Nucleoporin]]
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| - | [[Category: Nup58]]
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| - | [[Category: Nup88]]
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| - | [[Category: Nup93]]
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| - | [[Category: Protein transport]]
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| - | [[Category: Structural protein]]
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| - | [[Category: Triple helix]]
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