1sol

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A PIP2 AND F-ACTIN-BINDING SITE OF GELSOLIN, RESIDUE 150-169 (NMR, AVERAGED STRUCTURE)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The peptide G(150-169) corresponds to a phosphatidylinositol, 4,5-bisphosphate (PIP2) and filamentous actin (F-actin) binding site on, gelsolin (residues 150-169, with the sequence KHVVPNEVVVQRLFQVKGRR). The, conformation of this peptide in trifluoroethanol (TFE) aqueous solution, was determined by 1H nuclear magnetic resonance as the first step toward, understanding the structural aspects of the interaction of G(150-169) and, PIP2. The circular dichroism experiments show that G(150-169) adopts a, predominantly alpha-helical form in both 50% TFE aqueous solution and in, the presence of PIP2 micelles, therefore establishing a connection between, the two conformations. 1H nuclear magnetic resonance experiments of, G(150-169) in TFE co-solvent show that the helical region extends from, Pro-154 to Lys-166. The amphiphilic nature of this helical structure may, be the key to understanding the binding of the peptide to lipids. Sodium, dodecyl sulfate micelle solution is used as a model for anionic lipid, environments. Preliminary studies of the conformation of G(150-169) in, sodium dodecyl sulfate micelle solution show that the peptide forms an, alpha-helix similar to but with some structural differences from that in, TFE co-solvent. Fluorescence experiments provide evidence of peptide, clustering over a narrow range of peptide/PIP2 ratios, which is, potentially relevant to the biological function of PIP2.

Disease

Known disease associated with this structure: Amyloidosis, Finnish type OMIM:[137350]

About this Structure

1SOL is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Spectroscopic studies of a phosphoinositide-binding peptide from gelsolin: behavior in solutions of mixed solvent and anionic micelles., Xian W, Vegners R, Janmey PA, Braunlin WH, Biophys J. 1995 Dec;69(6):2695-702. PMID:8599675

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