3twe
From Proteopedia
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3twe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TWE FirstGlance]. <br> | <table><tr><td colspan='2'>[[3twe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TWE FirstGlance]. <br> | ||
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3twe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3twe OCA], [https://pdbe.org/3twe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3twe RCSB], [https://www.ebi.ac.uk/pdbsum/3twe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3twe ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3twe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3twe OCA], [https://pdbe.org/3twe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3twe RCSB], [https://www.ebi.ac.uk/pdbsum/3twe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3twe ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Noncanonical amino acids have proved extremely useful for modifying the properties of proteins. Among them, extensively fluorinated (fluorous) amino acids seem particularly effective in increasing protein stability; however, in the absence of structural data, the basis of this stabilizing effect remains poorly understood. To address this problem, we solved X-ray structures for three small proteins with hydrophobic cores that are packed with either fluorocarbon or hydrocarbon side chains and compared their stabilities. Although larger, the fluorinated residues are accommodated within the protein with minimal structural perturbation, because they closely match the shape of the hydrocarbon side chains that they replace. Thus, stability increases seem to be better explained by increases in buried hydrophobic surface area that accompany fluorination than by specific fluorous interactions between fluorinated side chains. This finding is illustrated by the design of a highly fluorinated protein that, by compensating for the larger volume and surface area of the fluorinated side chains, exhibits similar stability to its nonfluorinated counterpart. These structure-based observations should inform efforts to rationally modulate protein function using noncanonical amino acids. | ||
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| - | Structural basis for the enhanced stability of highly fluorinated proteins.,Buer BC, Meagher JL, Stuckey JA, Marsh EN Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4810-5. Epub 2012 Mar 12. PMID:22411812<ref>PMID:22411812</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3twe" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Buer | + | [[Category: Buer BC]] |
| - | [[Category: Marsh | + | [[Category: Marsh ENG]] |
| - | [[Category: Meagher | + | [[Category: Meagher JL]] |
| - | [[Category: Stuckey | + | [[Category: Stuckey JA]] |
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Current revision
Crystal Structure of the de novo designed peptide alpha4H
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