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| | <StructureSection load='3uer' size='340' side='right'caption='[[3uer]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='3uer' size='340' side='right'caption='[[3uer]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uer]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deigd Deigd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UER FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uer]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_geothermalis_DSM_11300 Deinococcus geothermalis DSM 11300]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UER FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z9N:'>Z9N</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900047:beta-turanose'>PRD_900047</scene>, <scene name='pdbligand=PRD_900081:alpha-turanose'>PRD_900081</scene>, <scene name='pdbligand=Z9N:alpha-D-fructofuranose'>Z9N</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ucq|3ucq]]</div></td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dgas, Dgeo_0572 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=319795 DEIGD])</td></tr>
| + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uer OCA], [https://pdbe.org/3uer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uer RCSB], [https://www.ebi.ac.uk/pdbsum/3uer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uer ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uer OCA], [https://pdbe.org/3uer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uer RCSB], [https://www.ebi.ac.uk/pdbsum/3uer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uer ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q1J0W0_DEIGD Q1J0W0_DEIGD] |
| - | Amylosucrases are sucrose-utilizing alpha-transglucosidases that naturally catalyze the synthesis of alpha-glucans, linked exclusively through alpha1,4-linkages. Side products and in particular sucrose isomers such as turanose and trehalulose are also produced by these enzymes. Here, we report the first structural and biophysical characterization of the most thermostable amylosucrase identified so far, the amylosucrase from Deinoccocus geothermalis (DgAS). The three-dimensional structure revealed a homodimeric quaternary organization, never reported before for other amylosucrases. A sequence signature of dimerization was identified from the analysis of the dimer interface and sequence alignments. By rigidifying the DgAS structure, the quaternary organization is likely to participate in the enhanced thermal stability of the protein. Amylosucrase specificity with respect to sucrose isomer formation (turanose or trehalulose) was also investigated. We report the first structures of the amylosucrases from Deinococcus geothermalis and Neisseria polysaccharea in complex with turanose. In the amylosucrase from N. polysaccharea (NpAS), key residues were found to force the fructosyl moiety to bind in an open state with the O3' ideally positioned to explain the preferential formation of turanose by NpAS. Such residues are either not present or not similarly placed in DgAS. As a consequence, DgAS binds the furanoid tautomers of fructose through a weak network of interactions to enable turanose formation. Such topology at subsite +1 is likely favoring other possible fructose binding modes in agreement with the higher amount of trehalulose formed by DgAS. Our findings help to understand the inter-relationships between amylosucrase structure, flexibility, function, and stability and provide new insight for amylosucrase design.
| + | |
| - | | + | |
| - | Structural Investigation of the Thermostability and Product Specificity of Amylosucrase from the Bacterium Deinococcus geothermalis.,Guerin F, Barbe S, Pizzut-Serin S, Potocki-Veronese G, Guieysse D, Guillet V, Monsan P, Mourey L, Remaud-Simeon M, Andre I, Tranier S J Biol Chem. 2012 Feb 24;287(9):6642-54. Epub 2011 Dec 29. PMID:22210773<ref>PMID:22210773</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3uer" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Amylosucrase]] | + | [[Category: Deinococcus geothermalis DSM 11300]] |
| - | [[Category: Deigd]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Andre, I]] | + | [[Category: Andre I]] |
| - | [[Category: Guerin, F]] | + | [[Category: Guerin F]] |
| - | [[Category: Guillet, V]] | + | [[Category: Guillet V]] |
| - | [[Category: Mourey, L]] | + | [[Category: Mourey L]] |
| - | [[Category: Pizzut-Serin, S]] | + | [[Category: Pizzut-Serin S]] |
| - | [[Category: Potocki-Veronese, G]] | + | [[Category: Potocki-Veronese G]] |
| - | [[Category: Remaud-Simeon, M]] | + | [[Category: Remaud-Simeon M]] |
| - | [[Category: Tranier, S]] | + | [[Category: Tranier S]] |
| - | [[Category: Alpha/beta-barrel]]
| + | |
| - | [[Category: Amylose synthesis]]
| + | |
| - | [[Category: Carbohydrate]]
| + | |
| - | [[Category: Glucosyltransferase]]
| + | |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Sucrose isomerization]]
| + | |
| - | [[Category: Thermostability]]
| + | |
| - | [[Category: Transferase]]
| + | |
