3ugm

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<StructureSection load='3ugm' size='340' side='right'caption='[[3ugm]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
<StructureSection load='3ugm' size='340' side='right'caption='[[3ugm]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ugm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanop Xanop]. The December 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TAL Effectors'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_12 10.2210/rcsb_pdb/mom_2014_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UGM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ugm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_oryzae_pv._oryzae_PXO99A Xanthomonas oryzae pv. oryzae PXO99A]. The December 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''TAL Effectors'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_12 10.2210/rcsb_pdb/mom_2014_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UGM FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pthXo1, PXO_00227 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=360094 XANOP])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ugm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ugm OCA], [https://pdbe.org/3ugm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ugm RCSB], [https://www.ebi.ac.uk/pdbsum/3ugm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ugm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ugm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ugm OCA], [https://pdbe.org/3ugm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ugm RCSB], [https://www.ebi.ac.uk/pdbsum/3ugm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ugm ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/PTHX1_XANOP PTHX1_XANOP] Avirulence protein. Acts as a transcription factor in rice, inducing expression of a number of host genes including SWEET11 (Os8N3, XA13, AC Q6YZF3) in susceptible plants with the Xa13 allele. Plants with the xa13 allele, which has an altered promoter, are resistant to bacterial blight caused by this bacterial strain and do not induce SWEET11. The xa13 allele elicits an atypical hypersensitive response (HR). PthXo1 binds SWEET11 promoter DNA in a sequence-specific manner.<ref>PMID:15553245</ref> <ref>PMID:16798873</ref> <ref>PMID:20345643</ref> <ref>PMID:22223736</ref>
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DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to 35 residues in length, that specify nucleotides via unique repeat variable diresidues (RVDs). The crystal structure of PthXo1 bound to its DNA target was determined using high-throughput computational structure prediction and validated by heavy-atom derivatization. Each repeat forms a left-handed, two-helix bundle that presents an RVD-containing loop to the DNA. The repeats self-associate to form a right-handed superhelix wrapped around the DNA major groove. The first RVD residue forms a stabilizing contact with the protein backbone, while the second makes a base-specific contact to the DNA sense strand. Two degenerate N-terminal repeats also interact with the DNA. Containing several RVDs and noncanonical associations, the structure illustrates the basis of TAL effector-DNA recognition.
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The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target.,Mak AN, Bradley P, Cernadas RA, Bogdanove AJ, Stoddard BL Science. 2012 Jan 5. PMID:22223736<ref>PMID:22223736</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ugm" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: TAL Effectors]]
[[Category: TAL Effectors]]
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[[Category: Xanop]]
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[[Category: Xanthomonas oryzae pv. oryzae PXO99A]]
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[[Category: Bogdanove, A J]]
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[[Category: Bogdanove AJ]]
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[[Category: Bradley, P]]
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[[Category: Bradley P]]
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[[Category: Cernadas, R A]]
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[[Category: Cernadas RA]]
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[[Category: Mak, A N.S]]
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[[Category: Mak ANS]]
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[[Category: Stoddard, B L]]
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[[Category: Stoddard BL]]
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[[Category: Dna binding protein]]
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[[Category: Tal effector]]
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[[Category: Transcription-dna complex]]
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Current revision

Structure of TAL effector PthXo1 bound to its DNA target

PDB ID 3ugm

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