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| | <StructureSection load='3uwx' size='340' side='right'caption='[[3uwx]], [[Resolution|resolution]] 4.40Å' scene=''> | | <StructureSection load='3uwx' size='340' side='right'caption='[[3uwx]], [[Resolution|resolution]] 4.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geos2 Geos2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_sp._Y412MC52 Geobacillus sp. Y412MC52]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UWX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.398Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GYMC52_3203, uvrA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550542 GEOS2]), GYMC52_3204, uvrB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550542 GEOS2])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwx OCA], [https://pdbe.org/3uwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwx RCSB], [https://www.ebi.ac.uk/pdbsum/3uwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwx ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uwx OCA], [https://pdbe.org/3uwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uwx RCSB], [https://www.ebi.ac.uk/pdbsum/3uwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uwx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/E8SW61_GEOS2 E8SW61_GEOS2]] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate (By similarity).[HAMAP-Rule:MF_00205] [[https://www.uniprot.org/uniprot/E8SW62_GEOS2 E8SW62_GEOS2]] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).[HAMAP-Rule:MF_00204][SAAS:SAAS004807_004_336891]
| + | [https://www.uniprot.org/uniprot/A0A0E0TG05_GEOS2 A0A0E0TG05_GEOS2] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.[HAMAP-Rule:MF_00205] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
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| - | Nucleotide excision repair (NER) is used by all organisms to eliminate DNA lesions. We determined the structure of the Geobacillus stearothermophilus UvrA-UvrB complex, the damage-sensor in bacterial NER and a new structure of UvrA. We observe that the DNA binding surface of UvrA, previously found in an open shape that binds damaged DNA, also exists in a closed groove shape compatible with native DNA only. The sensor contains two UvrB molecules that flank the UvrA dimer along the predicted path for DNA, ~80 A from the lesion. We show that the conserved signature domain II of UvrA mediates a nexus of contacts among UvrA, UvrB and DNA. Further, in our new structure of UvrA, this domain adopts an altered conformation while an adjacent nucleotide binding site is vacant. Our findings raise unanticipated questions about NER and also suggest a revised picture of its early stages.
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| - | Structure and mechanism of the UvrA-UvrB DNA damage sensor.,Pakotiprapha D, Samuels M, Shen K, Hu JH, Jeruzalmi D Nat Struct Mol Biol. 2012 Feb 5;19(3):291-8. doi: 10.1038/nsmb.2240. PMID:22307053<ref>PMID:22307053</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3uwx" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[UvrABC|UvrABC]] | | *[[UvrABC|UvrABC]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Geos2]] | + | [[Category: Geobacillus sp. Y412MC52]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jeruzalmi, D]] | + | [[Category: Jeruzalmi D]] |
| - | [[Category: Pakotiprapha, D]] | + | [[Category: Pakotiprapha D]] |
| - | [[Category: Abc atpase]]
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| - | [[Category: Atp binding]]
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| - | [[Category: Dna binding]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Dna repair]]
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| - | [[Category: Nucleotide excision repair]]
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| - | [[Category: Uvra]]
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| - | [[Category: Uvrb]]
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