4gx0
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4gx0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens_PCA Geobacter sulfurreducens PCA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GX0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4gx0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens_PCA Geobacter sulfurreducens PCA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GX0 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx0 OCA], [https://pdbe.org/4gx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gx0 RCSB], [https://www.ebi.ac.uk/pdbsum/4gx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx0 OCA], [https://pdbe.org/4gx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gx0 RCSB], [https://www.ebi.ac.uk/pdbsum/4gx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/Q74FS9_GEOSL Q74FS9_GEOSL] | [https://www.uniprot.org/uniprot/Q74FS9_GEOSL Q74FS9_GEOSL] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The gating ring-forming RCK domain regulates channel gating in response to various cellular chemical stimuli in eukaryotic Slo channel families and the majority of ligand-gated prokaryotic K(+) channels and transporters. Here we present structural and functional studies of a dual RCK-containing, multi-ligand gated K(+) channel from Geobacter sulfurreducens, named GsuK. We demonstrate that ADP and NAD(+) activate the GsuK channel, whereas Ca(2+) serves as an allosteric inhibitor. Multiple crystal structures elucidate the structural basis of multi-ligand gating in GsuK, and also reveal a unique ion conduction pore with segmented inner helices. Structural comparison leads us to propose a novel pore opening mechanics that is distinct from other K(+) channels.DOI:http://dx.doi.org/10.7554/eLife.00184.001. | ||
| - | |||
| - | Distinct gating mechanisms revealed by the structures of a multi-ligand gated K(+) channel.,Kong C, Zeng W, Ye S, Chen L, Sauer DB, Lam Y, Derebe MG, Jiang Y elife. 2012;1:e00184. doi: 10.7554/eLife.00184. Epub 2012 Dec 13. PMID:23240087<ref>PMID:23240087</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 4gx0" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of the GsuK L97D mutant
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Categories: Geobacter sulfurreducens PCA | Large Structures | Chen L | Derebe MG | Jiang Y | Kong C | Lam Y | Sauer DB | Ye S | Zeng W
