4hic

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [https://pdbe.org/4hic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [https://www.ebi.ac.uk/pdbsum/4hic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hic ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Conjugative plasmid transfer presents a serious threat to human health as the most important means of spreading antibiotic resistance and virulence genes among bacteria. The required direct cell-cell contact is established by a multi-protein complex, the conjugative type IV secretion system (T4SS). The conjugative core complex spans the cellular envelope and serves as a channel for macromolecular secretion. T4SSs of Gram-negative (G-) origin have been studied in great detail. In contrast, T4SSs of Gram-positive (G+) bacteria have only received little attention thus far, despite the medical relevance of numerous G+ pathogens (e.g. enterococci, staphylococci and streptococci). This study provides structural information on the type IV secretion (T4S) protein TraK of the G+ broad host range Enterococcus conjugative plasmid pIP501. The crystal structure of the N-terminally truncated construct TraKDelta was determined to 3.0 A resolution and exhibits a novel fold. Immunolocalization demonstrated that the protein localizes to the cell wall facing towards the cell exterior, but does not exhibit surface accessibility. Circular dichroism, dynamic light scattering and size-exclusion chromatography confirmed the protein to be a monomer. With the exception of proteins from closely related T4SSs, no significant sequence or structural relatives were found. This observation marks the protein as a very exclusive, specialized member of the pIP501 T4SS.

The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold.,Goessweiner-Mohr N, Fercher C, Arends K, Birner-Gruenberger R, Laverde-Gomez D, Huebner J, Grohmann E, Keller W Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1124-35. doi:, 10.1107/S1399004714001606. Epub 2014 Mar 21. PMID:24699656<ref>PMID:24699656</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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