4lo0

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lo0 OCA], [https://pdbe.org/4lo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lo0 RCSB], [https://www.ebi.ac.uk/pdbsum/4lo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lo0 ProSAT]</span></td></tr>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the fatal disease botulism, a flaccid paralysis of the muscle. BoNTs are released together with several auxiliary proteins as progenitor toxin complexes (PTCs) to become highly potent oral poisons. Here, we report the structure of a approximately 760 kDa 14-subunit large PTC of serotype A (L-PTC/A) and reveal insight into its absorption mechanism. Using a combination of X-ray crystallography, electron microscopy, and functional studies, we found that L-PTC/A consists of two structurally and functionally independent sub-complexes. A hetero-dimeric 290 kDa complex protects BoNT, while a hetero-dodecameric 470 kDa complex facilitates its absorption in the harsh environment of the gastrointestinal tract. BoNT absorption is mediated by nine glycan-binding sites on the dodecameric sub-complex that forms multivalent interactions with carbohydrate receptors on intestinal epithelial cells. We identified monosaccharides that blocked oral BoNT intoxication in mice, which suggests a new strategy for the development of preventive countermeasures for BoNTs based on carbohydrate receptor mimicry.

Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity.,Lee K, Gu S, Jin L, Le TT, Cheng LW, Strotmeier J, Kruel AM, Yao G, Perry K, Rummel A, Jin R PLoS Pathog. 2013 Oct;9(10):e1003690. doi: 10.1371/journal.ppat.1003690. Epub, 2013 Oct 10. PMID:24130488<ref>PMID:24130488</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 4lo0" style="background-color:#fffaf0;"></div>

== References ==

<references/>