4low

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>

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== Publication Abstract from PubMed ==

Carboxysomes are proteinaceous bacterial microcompartments that increase the efficiency of the rate-limiting step in carbon fixation by sequestering reaction substrates. Typically, alpha-carboxysomes are genetically encoded as a single operon expressing the microcompartment's structural proteins and its encapsulated enzymes. In addition, depending on phylogeny, as many as thirteen other genes are found to co-occur near or within alpha-carboxysome operons. One of these genes codes for a protein with distant homology to pterin-4a-carbinolamine dehydratase (PCD) enzymes. It is present in all alpha-carboxysome containing bacteria and has homologs in algae and higher plants. Canonical PCDs play an important role in amino acid hydroxylation, a reaction not associated with carbon fixation. We determined the crystal structure of an alpha-carboxysome PCD-like protein from the chemoautotrophic bacterium Thiomonas intermedia K12, at a resolution of 1.3 A. The protein retains a three-dimensional fold similar to canonical PCDs, though the prominent active site cleft present in PCD enzymes is disrupted in the alpha-carboxysome PCD-like protein. Using a cell-based complementation assay, we tested the PCD-like proteins from T. intermedia and two additional bacteria, and found no evidence for PCD enzymatic activity. However, we discovered that heterologous co-expression of the PCD-like protein from H. neapolitanus with RuBisCO and GroELS in E. coli increased the amount of soluble, assembled RuBisCO recovered from cell lysates compared to co-expression of RuBisCO with GroELS alone. We conclude that this conserved PCD-like protein, renamed here alpha carboxysome RuBisCO assembly factor (or acRAF), is a novel RuBisCO chaperone integral to alpha-carboxysome function.

Structure and identification of a pterin dehydratase-like protein as a RuBisCO assembly factor in the alpha-carboxysome.,Wheatley NM, Sundberg CD, Gidaniyan SD, Cascio D, Yeates TO J Biol Chem. 2014 Jan 23. PMID:24459150<ref>PMID:24459150</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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