1qni

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[[Image:1qni.jpg|left|200px]]
[[Image:1qni.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1qni |SIZE=350|CAPTION= <scene name='initialview01'>1qni</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1qni", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CUA:Cua+Center+(Electron+Entry+Gate)'>CUA</scene>, <scene name='pdbsite=CUB:Cua+Center+(Electron+Entry+Gate)'>CUB</scene>, <scene name='pdbsite=CUC:Cua+Center+(Electron+Entry+Gate)'>CUC</scene>, <scene name='pdbsite=CUD:Cua+Center+(Electron+Entry+Gate)'>CUD</scene>, <scene name='pdbsite=CUE:Cua+Center+(Electron+Entry+Gate)'>CUE</scene>, <scene name='pdbsite=CUF:Cua+Center+(Electron+Entry+Gate)'>CUF</scene>, <scene name='pdbsite=CUU:Cuz+Center+(Catalytic+Site)'>CUU</scene>, <scene name='pdbsite=CUV:Cuz+Center+(Catalytic+Site)'>CUV</scene>, <scene name='pdbsite=CUW:Cuz+Center+(Catalytic+Site)'>CUW</scene>, <scene name='pdbsite=CUX:Cuz+Center+(Catalytic+Site)'>CUX</scene>, <scene name='pdbsite=CUY:Cuz+Center+(Catalytic+Site)'>CUY</scene> and <scene name='pdbsite=CUZ:Cuz+Center+(Catalytic+Site)'>CUZ</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=CUZ:(MU-4-SULFIDO)-TETRA-NUCLEAR+COPPER+ION'>CUZ</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1qni| PDB=1qni | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qni OCA], [http://www.ebi.ac.uk/pdbsum/1qni PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qni RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION'''
'''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION'''
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[[Category: Cambillau, C.]]
[[Category: Cambillau, C.]]
[[Category: Tegoni, M.]]
[[Category: Tegoni, M.]]
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[[Category: crystal structure]]
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[[Category: Crystal structure]]
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[[Category: denitrification]]
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[[Category: Denitrification]]
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[[Category: electron transfer]]
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[[Category: Electron transfer]]
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[[Category: mad]]
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[[Category: Mad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:29:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:16:22 2008''
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Revision as of 03:29, 3 May 2008

Image:1qni.jpg

Template:STRUCTURE 1qni

CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM PSEUDOMONAS NAUTICA, AT 2.4A RESOLUTION


Overview

Nitrous oxide (N20) is a greenhouse gas, the third most significant contributor to global warming. As a key process for N20 elimination from the biosphere, N20 reductases catalyze the two-electron reduction of N20 to N2. These 2 x 65 kDa copper enzymes are thought to contain a CuA electron entry site, similar to that of cytochrome c oxidase, and a CuZ catalytic center. The copper anomalous signal was used to solve the crystal structure of N20 reductase from Pseudomonas nautica by multiwavelength anomalous dispersion, to a resolution of 2.4 A. The structure reveals that the CuZ center belongs to a new type of metal cluster, in which four copper ions are liganded by seven histidine residues. N20 binds to this center via a single copper ion. The remaining copper ions might act as an electron reservoir, assuring a fast electron transfer and avoiding the formation of dead-end products.

About this Structure

1QNI is a Single protein structure of sequence from Marinobacter hydrocarbonoclasticus. Full crystallographic information is available from OCA.

Reference

A novel type of catalytic copper cluster in nitrous oxide reductase., Brown K, Tegoni M, Prudencio M, Pereira AS, Besson S, Moura JJ, Moura I, Cambillau C, Nat Struct Biol. 2000 Mar;7(3):191-5. PMID:10700275 Page seeded by OCA on Sat May 3 06:29:15 2008

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