4rfs

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rfs OCA], [https://pdbe.org/4rfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rfs RCSB], [https://www.ebi.ac.uk/pdbsum/4rfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rfs ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Energy-coupling factor (ECF) transporters are a unique group of ATP-binding cassette (ABC) transporters responsible for micronutrient uptake from the environment. Each ECF transporter is composed of an S component (or EcfS protein) and T/A/A' components (or EcfT/A/A' proteins; ECF module). Among the group II ECF transporters, several EcfS proteins share one ECF module; however, the underlying mechanism remains unknown. Here we report the structure of a group II ECF transporter-pantothenate transporter from Lactobacillus brevis (LbECF-PanT), which shares the ECF module with the folate and hydroxymethylpyrimidine transporters (LbECF-FolT and LbECF-HmpT). Structural and mutational analyses revealed the residues constituting the pantothenate-binding pocket. We found that although the three EcfS proteins PanT, FolT, and HmpT are dissimilar in sequence, they share a common surface area composed of the transmembrane helices 1/2/6 (SM1/2/6) to interact with the coupling helices 2/3 (CH2/3) of the same EcfT. CH2 interacts mainly with SM1 via hydrophobic interactions, which may modulate the sliding movement of EcfS. CH3 binds to a hydrophobic surface groove formed by SM1, SM2, and SM6, which may transmit the conformational changes from EcfA/A' to EcfS. We also found that the residues at the intermolecular surfaces in LbECF-PanT are essential for transporter activity, and that these residues may mediate intermolecular conformational transmission and/or affect transporter complex stability. In addition, we found that the structure of EcfT is conformationally dynamic, which supports its function as a scaffold to mediate the interaction of the ECF module with various EcfS proteins to form different transporter complexes.

Structure of a pantothenate transporter and implications for ECF module sharing and energy coupling of group II ECF transporters.,Zhang M, Bao Z, Zhao Q, Guo H, Xu K, Wang C, Zhang P Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18560-5. doi:, 10.1073/pnas.1412246112. Epub 2014 Dec 15. PMID:25512487<ref>PMID:25512487</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

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