7sez

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Current revision (13:06, 1 March 2024) (edit) (undo)
 
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<StructureSection load='7sez' size='340' side='right'caption='[[7sez]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='7sez' size='340' side='right'caption='[[7sez]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7sez]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SEZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7sez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_Western_Reserve Vaccinia virus Western Reserve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SEZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8NK:7-METHYLGUANOSINE+5-DIPHOSPHATE'>8NK</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7000124&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8NK:7-METHYLGUANOSINE+5-DIPHOSPHATE'>8NK</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sez OCA], [https://pdbe.org/7sez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sez RCSB], [https://www.ebi.ac.uk/pdbsum/7sez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sez ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sez OCA], [https://pdbe.org/7sez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sez RCSB], [https://www.ebi.ac.uk/pdbsum/7sez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sez ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/L7QJE0_9POXV L7QJE0_9POXV]] Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis. Does not cleave unmethylated RNAs or RNAs shorter than 24 nucleotides.[ARBA:ARBA00025519]
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[https://www.uniprot.org/uniprot/D9_VACCW D9_VACCW]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Poxviruses encode decapping enzymes that remove the protective 5' cap from both host and viral mRNAs to commit transcripts for decay by the cellular exonuclease Xrn1. Decapping by these enzymes is critical for poxvirus pathogenicity by means of simultaneously suppressing host protein synthesis and limiting the accumulation of viral double-stranded RNA (dsRNA), a trigger for antiviral responses. Here we present a high-resolution structural view of the vaccinia virus decapping enzyme D9. This Nudix enzyme contains a domain organization different from other decapping enzymes in which a three-helix bundle is inserted into the catalytic Nudix domain. The 5' mRNA cap is positioned in a bipartite active site at the interface of the two domains. Specificity for the methylated guanosine cap is achieved by stacking between conserved aromatic residues in a manner similar to that observed in canonical cap-binding proteins VP39, eIF4E, and CBP20, and distinct from eukaryotic decapping enzyme Dcp2.
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysis.,Peters JK, Tibble RW, Warminski M, Jemielity J, Gross JD Structure. 2022 Mar 2. pii: S0969-2126(22)00048-X. doi:, 10.1016/j.str.2022.02.012. PMID:35290794<ref>PMID:35290794</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7sez" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Gross, J D]]
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[[Category: Vaccinia virus Western Reserve]]
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[[Category: Jemielity, J]]
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[[Category: Gross JD]]
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[[Category: Peters, J K]]
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[[Category: Jemielity J]]
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[[Category: Tibble, R W]]
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[[Category: Peters JK]]
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[[Category: Warminski, M]]
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[[Category: Tibble RW]]
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[[Category: Decapping]]
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[[Category: Warminski M]]
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[[Category: Mrna cap]]
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[[Category: Nudix]]
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[[Category: Viral protein]]
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Current revision

Crystal structure of Vaccinia Virus decapping enzyme D9 in complex with m7GDP

PDB ID 7sez

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