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7t7c

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Current revision (13:07, 1 March 2024) (edit) (undo)
 
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<StructureSection load='7t7c' size='340' side='right'caption='[[7t7c]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
<StructureSection load='7t7c' size='340' side='right'caption='[[7t7c]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7t7c]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T7C FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7t7c]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T7C FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t7c OCA], [https://pdbe.org/7t7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t7c RCSB], [https://www.ebi.ac.uk/pdbsum/7t7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t7c ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t7c OCA], [https://pdbe.org/7t7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t7c RCSB], [https://www.ebi.ac.uk/pdbsum/7t7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t7c ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A822AJ50_9NEOB A0A822AJ50_9NEOB]
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How can exactly six SNARE complexes be assembled under each synaptic vesicle? Here we report cryo-EM crystal structures of the core domain of Munc13, the key chaperone that initiates SNAREpin assembly. The functional core of Munc13, consisting of C1-C2B-MUN-C2C (Munc13C) spontaneously crystallizes between phosphatidylserine-rich bilayers in two distinct conformations, each in a radically different oligomeric state. In the open conformation (state 1), Munc13C forms upright trimers that link the two bilayers, separating them by approximately 21 nm. In the closed conformation, six copies of Munc13C interact to form a lateral hexamer elevated approximately 14 nm above the bilayer. Open and closed conformations differ only by a rigid body rotation around a flexible hinge, which when performed cooperatively assembles Munc13 into a lateral hexamer (state 2) in which the key SNARE assembly-activating site of Munc13 is autoinhibited by its neighbor. We propose that each Munc13 in the lateral hexamer ultimately assembles a single SNAREpin, explaining how only and exactly six SNARE complexes are templated. We suggest that state 1 and state 2 may represent two successive states in the synaptic vesicle supply chain leading to "primed" ready-release vesicles in which SNAREpins are clamped and ready to release (state 3).
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Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release.,Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE Proc Natl Acad Sci U S A. 2022 Feb 15;119(7). pii: 2121259119. doi:, 10.1073/pnas.2121259119. PMID:35135883<ref>PMID:35135883</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7t7c" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Grushin, K]]
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[[Category: Rattus norvegicus]]
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[[Category: Sindelar, C V]]
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[[Category: Grushin K]]
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[[Category: Exocytosis]]
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[[Category: Sindelar CV]]
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[[Category: Membrane fusion]]
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[[Category: Munc13]]
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[[Category: Synaptic transmission]]
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Current revision

The hexagonal organization of Munc13-1 C1-C2B-MUN-C2C domains between lipid bilayers

PDB ID 7t7c

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