This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


7tfc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:08, 1 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='7tfc' size='340' side='right'caption='[[7tfc]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
<StructureSection load='7tfc' size='340' side='right'caption='[[7tfc]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[7tfc]] is a 28 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFC FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[7tfc]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TFC FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfc OCA], [https://pdbe.org/7tfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfc RCSB], [https://www.ebi.ac.uk/pdbsum/7tfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tfc OCA], [https://pdbe.org/7tfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tfc RCSB], [https://www.ebi.ac.uk/pdbsum/7tfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tfc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[https://www.uniprot.org/uniprot/GLNR_BACSU GLNR_BACSU]] Represses many genes including the glnRA operon and tnrA during growth with nitrogen excess.<ref>PMID:10231480</ref> <ref>PMID:1677938</ref> <ref>PMID:2573733</ref>
+
[https://www.uniprot.org/uniprot/A0A085CCI2_BACIU A0A085CCI2_BACIU]
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
How bacteria sense and respond to nitrogen levels are central questions in microbial physiology. In Gram-positive bacteria, nitrogen homeostasis is controlled by an operon encoding glutamine synthetase (GS), a dodecameric machine that assimilates ammonium into glutamine, and the GlnR repressor. GlnR detects nitrogen excess indirectly by binding glutamine-feedback-inhibited-GS (FBI-GS), which activates its transcription-repression function. The molecular mechanisms behind this regulatory circuitry, however, are unknown. Here we describe biochemical and structural analyses of GS and FBI-GS-GlnR complexes from pathogenic and non-pathogenic Gram-positive bacteria. The structures show FBI-GS binds the GlnR C-terminal domain within its active-site cavity, juxtaposing two GlnR monomers to form a DNA-binding-competent GlnR dimer. The FBI-GS-GlnR interaction stabilizes the inactive GS conformation. Strikingly, this interaction also favors a remarkable dodecamer to tetradecamer transition in some GS, breaking the paradigm that all bacterial GS are dodecamers. These data thus unveil unique structural mechanisms of transcription and enzymatic regulation.
+
-
Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria.,Travis BA, Peck JV, Salinas R, Dopkins B, Lent N, Nguyen VD, Borgnia MJ, Brennan RG, Schumacher MA Nat Commun. 2022 Jul 1;13(1):3793. doi: 10.1038/s41467-022-31573-0. PMID:35778410<ref>PMID:35778410</ref>
+
==See Also==
-
 
+
*[[Glutamine synthetase 3D structures|Glutamine synthetase 3D structures]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 7tfc" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Glutamate--ammonia ligase]]
+
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Peck, J]]
+
[[Category: Peck J]]
-
[[Category: Schumacher, M A]]
+
[[Category: Schumacher MA]]
-
[[Category: Travis, B A]]
+
[[Category: Travis BA]]
-
[[Category: Biosynthetic protein]]
+
-
[[Category: Glutamine synthetase repressor tetradecamer]]
+
-
[[Category: Ligase]]
+

Current revision

B. subtilis GS(14)-Q-GlnR peptide

PDB ID 7tfc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7tfc"
Personal tools