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3pjz

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Current revision (13:32, 1 March 2024) (edit) (undo)
 
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<StructureSection load='3pjz' size='340' side='right'caption='[[3pjz]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
<StructureSection load='3pjz' size='340' side='right'caption='[[3pjz]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PJZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pjz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PJZ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.506&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP0032 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pjz OCA], [https://pdbe.org/3pjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pjz RCSB], [https://www.ebi.ac.uk/pdbsum/3pjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pjz ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pjz OCA], [https://pdbe.org/3pjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pjz RCSB], [https://www.ebi.ac.uk/pdbsum/3pjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pjz ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/TRKH_VIBPA TRKH_VIBPA]] Low-affinity potassium transport system. Interacts with trk system potassium uptake protein TrkA and requires TrkE for transport activity. Selective for permeation of potassium ion and rubidium ion over smaller ions such as natrium or litium.
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[https://www.uniprot.org/uniprot/TRKH_VIBPA TRKH_VIBPA] Low-affinity potassium transport system. Interacts with trk system potassium uptake protein TrkA and requires TrkE for transport activity. Selective for permeation of potassium ion and rubidium ion over smaller ions such as natrium or litium.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The TrkH/TrkG/KtrB proteins mediate K(+) uptake in bacteria and probably evolved from simple K(+) channels by multiple gene duplications or fusions. Here we present the crystal structure of a TrkH from Vibrio parahaemolyticus. TrkH is a homodimer, and each protomer contains an ion permeation pathway. A selectivity filter, similar in architecture to those of K(+) channels but significantly shorter, is lined by backbone and side-chain oxygen atoms. Functional studies showed that TrkH is selective for permeation of K(+) and Rb(+) over smaller ions such as Na(+) or Li(+). Immediately intracellular to the selectivity filter are an intramembrane loop and an arginine residue, both highly conserved, which constrict the permeation pathway. Substituting the arginine with an alanine significantly increases the rate of K(+) flux. These results reveal the molecular basis of K(+) selectivity and suggest a novel gating mechanism for this large and important family of membrane transport proteins.
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Crystal structure of a potassium ion transporter, TrkH.,Cao Y, Jin X, Huang H, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M Nature. 2011 Feb 13. PMID:21317882<ref>PMID:21317882</ref>
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==See Also==
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*[[Tyrosine kinase receptor 3D structures|Tyrosine kinase receptor 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pjz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cao, Y]]
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[[Category: Vibrio parahaemolyticus]]
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[[Category: Huang, H]]
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[[Category: Cao Y]]
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[[Category: Jin, X]]
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[[Category: Huang H]]
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[[Category: Levin, E J]]
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[[Category: Jin X]]
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[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
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[[Category: Levin EJ]]
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[[Category: Zhou, M]]
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[[Category: Zhou M]]
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[[Category: Membrane]]
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[[Category: New york consortium on membrane protein structure]]
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[[Category: Nycomp]]
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[[Category: Potassium transport]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Structural genomic]]
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[[Category: Transport protein]]
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Current revision

Crystal Structure of the Potassium Transporter TrkH from Vibrio parahaemolyticus

PDB ID 3pjz

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