1qrt

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[[Image:1qrt.gif|left|200px]]
[[Image:1qrt.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1qrt |SIZE=350|CAPTION= <scene name='initialview01'>1qrt</scene>, resolution 2.700&Aring;
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The line below this paragraph, containing "STRUCTURE_1qrt", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamine--tRNA_ligase Glutamine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.18 6.1.1.18] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TRNAGLN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), GLNS10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1qrt| PDB=1qrt | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qrt OCA], [http://www.ebi.ac.uk/pdbsum/1qrt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qrt RCSB]</span>
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}}
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'''GLUTAMINYL-TRNA SYNTHETASE MUTANT D235G COMPLEXED WITH GLUTAMINE TRANSFER RNA'''
'''GLUTAMINYL-TRNA SYNTHETASE MUTANT D235G COMPLEXED WITH GLUTAMINE TRANSFER RNA'''
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[[Category: Arnez, J G.]]
[[Category: Arnez, J G.]]
[[Category: Steitz, T A.]]
[[Category: Steitz, T A.]]
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[[Category: aminoacyl-trna synthase]]
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[[Category: Aminoacyl-trna synthase]]
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[[Category: atp-b]]
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[[Category: Atp-b]]
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[[Category: complex (aminoacyl-trna synthase/trna)]]
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[[Category: Ligase]]
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[[Category: ligase]]
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[[Category: Protein biosynthesis]]
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[[Category: protein biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:37:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:18:06 2008''
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Revision as of 03:37, 3 May 2008

Image:1qrt.gif

Template:STRUCTURE 1qrt

GLUTAMINYL-TRNA SYNTHETASE MUTANT D235G COMPLEXED WITH GLUTAMINE TRANSFER RNA


Overview

Three previously described mutant Escherichia coli glutaminyl-tRNA synthetase (GlnRS) proteins that incorrectly aminoacylate the amber suppressor derived from tRNATyr (supF) with glutamine were cocrystallized with wild-type tRNAGln and their structures determined. In two of the mutant enzymes studied, Asp235, which contacts base pair G3-C70 in the acceptor stem, has been changed to asparagine in GlnRS7 and to glycine in GlnRS10. These mutations result in changed interactions between Asn235 of GlnRS7 and G3-C70 of the tRNA and an altered water structure between Gly235 of GlnRS10 and base pair G3-C70. These structures suggest how the mutant enzymes can show only small changes in their ability to aminoacylate wild-type cognate tRNA on the one hand and yet show a lack of discrimination against a noncognate U3-A70 base pair on the other. In contrast, the change of Ile129 to Thr in GlnRS15 causes virtually no change in the structure of the complex, and the explanation for its ability to misacylate supF is unclear.

About this Structure

1QRT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of three misacylating mutants of Escherichia coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP., Arnez JG, Steitz TA, Biochemistry. 1996 Nov 26;35(47):14725-33. PMID:8942633 Page seeded by OCA on Sat May 3 06:37:54 2008

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