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| | <StructureSection load='5t58' size='340' side='right'caption='[[5t58]], [[Resolution|resolution]] 3.21Å' scene=''> | | <StructureSection load='5t58' size='340' side='right'caption='[[5t58]], [[Resolution|resolution]] 3.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5t58]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T58 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5T58 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5t58]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T58 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2131Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5t51|5t51]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t58 OCA], [https://pdbe.org/5t58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t58 RCSB], [https://www.ebi.ac.uk/pdbsum/5t58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t58 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0_F02343g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_E05809g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_D15741g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_C15939g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5t58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t58 OCA], [http://pdbe.org/5t58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t58 RCSB], [http://www.ebi.ac.uk/pdbsum/5t58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t58 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q6CLK3_KLULA Q6CLK3_KLULA] |
| - | Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
| + | |
| - | | + | |
| - | Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5t58" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candida sphaerica]] | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dimitrova, Y]] | + | [[Category: Dimitrova Y]] |
| - | [[Category: Harrison, S C]] | + | [[Category: Harrison SC]] |
| - | [[Category: Jenni, S]] | + | [[Category: Jenni S]] |
| - | [[Category: Khin, Y]] | + | [[Category: Khin Y]] |
| - | [[Category: Valverde, R]] | + | [[Category: Valverde R]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Chromosome]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Kinetochore]]
| + | |
| - | [[Category: Mind]]
| + | |
| - | [[Category: Mis12]]
| + | |
| - | [[Category: Mtw1 complex]]
| + | |
| - | [[Category: Segregation]]
| + | |
