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| | <StructureSection load='5thq' size='340' side='right'caption='[[5thq]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5thq' size='340' side='right'caption='[[5thq]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5thq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5THQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5thq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5THQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5THQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tii|5tii]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">arx21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=77133 uncultivated bacterium])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5thq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5thq OCA], [https://pdbe.org/5thq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5thq RCSB], [https://www.ebi.ac.uk/pdbsum/5thq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5thq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5thq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5thq OCA], [http://pdbe.org/5thq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5thq RCSB], [http://www.ebi.ac.uk/pdbsum/5thq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5thq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A023PKG5_9BACT A0A023PKG5_9BACT] |
| - | Arixanthomycins are pentangular polyphenols (PP) with potent antiproliferative activities that were discovered through the heterologous expression of environmental DNA-derived gene clusters. The biosynthesis of arixanthomycin and other PPs is unusual, because it requires several novel type II polyketide synthase (PKS) enzymes for its complete maturation. Most type II PKSs contain a ketoreductase (KR) that mediates the C7-C12 first ring cyclization and C9-reduction. In contrast, based on previous studies of product analysis and genome mining, the arixanthomycin (ARX) gene cluster like harbors a C11-reducing ketoreductase (ARX 27), a C9-C14 first-ring aromatase/cyclase (ARX 19), and an unprecedented C-17 and C-19 reducing KR (ARX 21). While bioinformatics is useful for predicting novel enzymes, the functions of ARX 19, ARX 21 and ARX 27 have yet to be confirmed. Further, the structural features that predispose the ARX biosynthetic enzymes to process atypical poly-beta-ketone scaffolds remain unknown. We report the crystal structure of ARX 21, the first structure of an enzyme involved in PP biosynthesis and likely a C17 and C19 reducing-KR, which is structurally similar to C-15 reducing KRs. Structural comparison of ARX 21 and other C9-reducing KRs revealed difference in the enzyme activity site that may enlighten the molecular basis of KR substrate specificity. In addition, we report the successful in vitro reconstitution of ARX 19. The structural characterization of ARX 21 in conjunction with the in vitro results of ARX 19 lays the groundwork towards a complete in vitro and structural characterization of type II PKS enzymes involved in PP biogenesis.
| + | |
| - | | + | |
| - | Comprehensive Analysis of a Novel Ketoreductase for Pentangular Polyphenol Biosynthesis.,Valentic TR, Jackson DR, Brady SF, Tsai SS ACS Chem Biol. 2016 Oct 25. PMID:27779377<ref>PMID:27779377</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5thq" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Uncultivated bacterium]] | + | [[Category: Uncultured bacterium]] |
| - | [[Category: Brady, S F]] | + | [[Category: Brady SF]] |
| - | [[Category: Tsai, S C]] | + | [[Category: Tsai SC]] |
| - | [[Category: Valentic, T R]] | + | [[Category: Valentic TR]] |
| - | [[Category: Arixanthomycin]]
| + | |
| - | [[Category: Ketoreduction]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Pentangular polyphenol polyketide]]
| + | |
