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| | <StructureSection load='5tsz' size='340' side='right'caption='[[5tsz]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='5tsz' size='340' side='right'caption='[[5tsz]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tsz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TSZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tsz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TSZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.002Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pvCelTOS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5855 Haemamoeba vivax])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsz OCA], [http://pdbe.org/5tsz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tsz RCSB], [http://www.ebi.ac.uk/pdbsum/5tsz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsz ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsz OCA], [https://pdbe.org/5tsz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tsz RCSB], [https://www.ebi.ac.uk/pdbsum/5tsz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q53UB7_PLAVI Q53UB7_PLAVI] |
| - | Apicomplexan parasites contain a conserved protein CelTOS that, in malaria parasites, is essential for traversal of cells within the mammalian host and arthropod vector. However, the molecular role of CelTOS is unknown because it lacks sequence similarity to proteins of known function. Here, we determined the crystal structure of CelTOS and discovered CelTOS resembles proteins that bind to and disrupt membranes. In contrast to known membrane disruptors, CelTOS has a distinct architecture, specifically binds phosphatidic acid commonly present within the inner leaflet of plasma membranes, and potently disrupts liposomes composed of phosphatidic acid by forming pores. Microinjection of CelTOS into cells resulted in observable membrane damage. Therefore, CelTOS is unique as it achieves nearly universal inner leaflet cellular activity to enable the exit of parasites from cells during traversal. By providing novel molecular insight into cell traversal by apicomplexan parasites, our work facilitates the design of therapeutics against global pathogens.
| + | |
| - | | + | |
| - | Malaria parasite CelTOS targets the inner leaflet of cell membranes for pore-dependent disruption.,Jimah JR, Salinas ND, Sala-Rabanal M, Jones NG, Sibley LD, Nichols CG, Schlesinger PH, Tolia NH Elife. 2016 Dec 1;5. pii: e20621. doi: 10.7554/eLife.20621. PMID:27906127<ref>PMID:27906127</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5tsz" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Haemamoeba vivax]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jimah, J R]] | + | [[Category: Plasmodium vivax]] |
| - | [[Category: Tolia, N H]] | + | [[Category: Jimah JR]] |
| - | [[Category: Cell invasion]] | + | [[Category: Tolia NH]] |
| - | [[Category: Malaria]]
| + | |
| - | [[Category: Membrane disruption]]
| + | |
| - | [[Category: Pore]]
| + | |
| - | [[Category: Traversal]]
| + | |
