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| | ==Crystal structure of S. aureus TarS in complex with UDP-GlcNAc== | | ==Crystal structure of S. aureus TarS in complex with UDP-GlcNAc== |
| - | <StructureSection load='5tze' size='340' side='right' caption='[[5tze]], [[Resolution|resolution]] 2.33Å' scene=''> | + | <StructureSection load='5tze' size='340' side='right'caption='[[5tze]], [[Resolution|resolution]] 2.33Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tze]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TZE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TZE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tze]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TZE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TZE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.33Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tzk|5tzk]], [[5tz8|5tz8]], [[5tzj|5tzj]], [[5tzi|5tzi]], [[5u02|5u02]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAMEA2236484_00765, SAMEA2384030_00583, SAMEA2384487_01253, SAMEA2445549_00791, SAMEA2445572_00749, SAMEA2445608_00472, SAMEA2445616_00196, SAMEA2445622_01196, SAMEA2445624_00746, SAMEA2445630_01744, SAMEA2445663_00417, SAMEA2445672_00193 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tze OCA], [https://pdbe.org/5tze PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tze RCSB], [https://www.ebi.ac.uk/pdbsum/5tze PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tze ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tze FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tze OCA], [http://pdbe.org/5tze PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tze RCSB], [http://www.ebi.ac.uk/pdbsum/5tze PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tze ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/TARS_STAAM TARS_STAAM] Attaches beta-O-GlcNAc (beta-O-N-acetyl-D-glucosamine) residues to the C4 position of poly(RboP)-wall teichoic acids (WTAs). Mediates beta-lactam resistance in methicillin resistant Staphylococcus aureus (MRSA) strains.<ref>PMID:27973583</ref> |
| - | In recent years, there has been a growing interest in teichoic acids as targets for antibiotic drug design against major clinical pathogens such as Staphylococcus aureus, reflecting the disquieting increase in antibiotic resistance and the historical success of bacterial cell wall components as drug targets. It is now becoming clear that beta-O-GlcNAcylation of S. aureus wall teichoic acids plays a major role in both pathogenicity and antibiotic resistance. Here we present the first structure of S. aureus TarS, the enzyme responsible for polyribitol phosphate beta-O-GlcNAcylation. Using a divide and conquer strategy, we obtained crystal structures of various TarS constructs, mapping high resolution overlapping N-terminal and C-terminal structures onto a lower resolution full-length structure that resulted in a high resolution view of the entire enzyme. Using the N-terminal structure that encapsulates the catalytic domain, we furthermore captured several snapshots of TarS, including the native structure, the UDP-GlcNAc donor complex, and the UDP product complex. These structures along with structure-guided mutants allowed us to elucidate various catalytic features and identify key active site residues and catalytic loop rearrangements that provide a valuable platform for anti-MRSA drug design. We furthermore observed for the first time the presence of a trimerization domain composed of stacked carbohydrate binding modules, commonly observed in starch active enzymes, but adapted here for a poly sugar-phosphate glycosyltransferase.
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| - | Structure and Mechanism of Staphylococcus aureus TarS, the Wall Teichoic Acid beta-glycosyltransferase Involved in Methicillin Resistance.,Sobhanifar S, Worrall LJ, King DT, Wasney GA, Baumann L, Gale RT, Nosella M, Brown ED, Withers SG, Strynadka NC PLoS Pathog. 2016 Dec 14;12(12):e1006067. doi: 10.1371/journal.ppat.1006067., eCollection 2016 Dec. PMID:27973583<ref>PMID:27973583</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5tze" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: King, D T]] | + | [[Category: Large Structures]] |
| - | [[Category: Sobhanifar, S]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Strynadka, N C]] | + | [[Category: King DT]] |
| - | [[Category: Worrall, L J]] | + | [[Category: Sobhanifar S]] |
| - | [[Category: Glycosyltransferase]] | + | [[Category: Strynadka NC]] |
| - | [[Category: Gt-a]] | + | [[Category: Worrall LJ]] |
| - | [[Category: Transferase]]
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| - | [[Category: Wall teichoic acid]]
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