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5uki

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==Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1==
==Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1==
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<StructureSection load='5uki' size='340' side='right' caption='[[5uki]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='5uki' size='340' side='right'caption='[[5uki]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5uki]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UKI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5uki]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UKI FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_062730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uki OCA], [http://pdbe.org/5uki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uki RCSB], [http://www.ebi.ac.uk/pdbsum/5uki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uki ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uki OCA], [https://pdbe.org/5uki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uki RCSB], [https://www.ebi.ac.uk/pdbsum/5uki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uki ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C4M1P9_ENTH1 C4M1P9_ENTH1]
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The RNA lariat debranching enzyme, Dbr1, is a metallophosphoesterase that cleaves 2'-5' phosphodiester bonds within intronic lariats. Previous reports have indicated that Dbr1 enzymatic activity is supported by diverse metal ions including Ni(2+) , Mn(2+) , Mg(2+) , Fe(2+) , and Zn(2+) . While in initial structures of the Entamoeba histolytica Dbr1 only one of the two catalytic metal-binding sites were observed to be occupied (with a Mn(2+) ion), recent structures determined a Zn(2+) /Fe(2+) heterobinucleation. We solved a high-resolution X-ray crystal structure (1.8 A) of the E. histolytica Dbr1 and determined a Zn(2+) /Mn(2+) occupancy. ICP-AES corroborate this finding, and in vitro debranching assays with fluorescently labeled branched substrates confirm activity.
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Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn(2+) /Mn(2+) heterobinucleation.,Ransey E, Paredes E, Dey SK, Das SR, Heroux A, Macbeth MR FEBS Lett. 2017 Jul;591(13):2003-2010. doi: 10.1002/1873-3468.12677. Epub 2017, Jun 14. PMID:28504306<ref>PMID:28504306</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5uki" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Enthi]]
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[[Category: Entamoeba histolytica]]
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[[Category: Macbeth, M R]]
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[[Category: Large Structures]]
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[[Category: Ransey, L]]
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[[Category: Macbeth MR]]
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[[Category: Debranching]]
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[[Category: Ransey L]]
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[[Category: Hydrolase]]
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[[Category: Metal ion]]
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[[Category: Rna]]
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Current revision

Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1

PDB ID 5uki

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