This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5uqo

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of 2-methylcitrate synthase from Aspergillus fumigatus

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5uqo"

Categories: Aspergillus fumigatus | Large Structures | Chruszcz M | Schlachter C

@@ Line 3: / Line 3: @@
 <StructureSection load='5uqo' size='340' side='right'caption='[[5uqo]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5uqo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UQO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5UQO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5uqo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UQO FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uqs|5uqs]], [[5uqq|5uqq]], [[5uqr|5uqr]], [[5uqu|5uqu]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mcsA, cit1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uqo OCA], [https://pdbe.org/5uqo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uqo RCSB], [https://www.ebi.ac.uk/pdbsum/5uqo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uqo ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5uqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uqo OCA], [http://pdbe.org/5uqo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uqo RCSB], [http://www.ebi.ac.uk/pdbsum/5uqo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uqo ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PRPC_ASPFM PRPC_ASPFM]] Component of the methylcitrate cycle that catalyzes the synthesis of (2S,3S)-2-methylcitrate from propionyl-CoA and oxaloacetate. Plays an important role in detoxification of propionyl-CoA, an inhibitor of both primary and secondary metabolism. Has also citrate synthase activity using as substrates acetyl-CoA and oxaloacetate. May play a role in virulence.<ref>PMID:16008561</ref>
+[https://www.uniprot.org/uniprot/PRPC_ASPFM PRPC_ASPFM] Component of the methylcitrate cycle that catalyzes the synthesis of (2S,3S)-2-methylcitrate from propionyl-CoA and oxaloacetate. Plays an important role in detoxification of propionyl-CoA, an inhibitor of both primary and secondary metabolism. Has also citrate synthase activity using as substrates acetyl-CoA and oxaloacetate. May play a role in virulence.<ref>PMID:16008561</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Aspergillus fumigatus is a ubiquitous fungus that is not only a problem in agriculture, but also in healthcare. Aspergillus fumigatus drug resistance is becoming more prominent which is mainly attributed to the widespread use of fungicides in agriculture. The fungi-specific 2-methylcitrate cycle is responsible for detoxifying propionyl-CoA, a toxic metabolite produced as the fungus breaks down proteins and amino acids. The enzyme responsible for this detoxification is 2-methylcitrate synthase (mcsA) and is a potential candidate for the design of new anti-fungals. However, mcsA is very similar in structure to human citrate synthase (hCS) and catalyzes the same reaction. Therefore, both enzymes were studied in parallel to provide foundations for design of mcsA-specific inhibitors. The first crystal structures of citrate synthase from humans and 2-methylcitrate synthase from A. fumigatus are reported. The determined structures capture various conformational states of the enzymes and several inhibitors were identified and characterized. Despite a significant homology, mcsA and hCS display pronounced differences in substrate specificity and cooperativity. Considering that the active sites of the enzymes are almost identical, the differences in reactions catalyzed by enzymes are caused by residues that are in the vicinity of the active site and influence conformational changes of the enzymes.
-Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.,Schlachter CR, Klapper V, Radford T, Chruszcz M Biol Chem. 2019 Nov 26;400(12):1567-1581. doi: 10.1515/hsz-2019-0106. PMID:31141475<ref>PMID:31141475</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 5uqo" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 27: / Line 17: @@
 __TOC__
 </StructureSection>
-[[Category: Aspfm]]
+[[Category: Aspergillus fumigatus]]
 [[Category: Large Structures]]
-[[Category: Chruszcz, M]]
+[[Category: Chruszcz M]]
-[[Category: Schlachter, C]]
+[[Category: Schlachter C]]
-[[Category: 2-methylcitrate synthase]]
-[[Category: Citrate synthase]]
-[[Category: Mcsa]]
-[[Category: Transferase]]

5uqo

From Proteopedia

Current revision

Crystal structure of 2-methylcitrate synthase from Aspergillus fumigatus

Structural highlights

Function

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox