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| | <StructureSection load='5uw7' size='340' side='right'caption='[[5uw7]], [[Resolution|resolution]] 2.37Å' scene=''> | | <StructureSection load='5uw7' size='340' side='right'caption='[[5uw7]], [[Resolution|resolution]] 2.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5uw7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5UW7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5uw7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gypsophila_vaccaria Gypsophila vaccaria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UW7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.37Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5uw3|5uw3]], [[5uw5|5uw5]], [[5uw6|5uw6]], [[5uzw|5uzw]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5uw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw7 OCA], [http://pdbe.org/5uw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uw7 RCSB], [http://www.ebi.ac.uk/pdbsum/5uw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uw7 OCA], [https://pdbe.org/5uw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uw7 RCSB], [https://www.ebi.ac.uk/pdbsum/5uw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uw7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/R4P353_GYPVA R4P353_GYPVA] |
| - | Enzymes that can catalyze the macrocyclization of linear peptide substrates have long been sought for the production of libraries of structurally diverse scaffolds via combinatorial gene assembly as well as to afford rapid in vivo screening methods. Orbitides are plant ribosomally synthesized and posttranslationally modified peptides (RiPPs) of various sizes and topologies, several of which are shown to be biologically active. The diversity in size and sequence of orbitides suggests that the corresponding macrocyclases may be ideal catalysts for production of cyclic peptides. Here we present the biochemical characterization and crystal structures of the plant enzyme PCY1 involved in orbitide macrocyclization. These studies demonstrate how the PCY1 S9A protease fold has been adapted for transamidation, rather than hydrolysis, of acyl-enzyme intermediates to yield cyclic products. Notably, PCY1 uses an unusual strategy in which the cleaved C-terminal follower peptide from the substrate stabilizes the enzyme in a productive conformation to facilitate macrocyclization of the N-terminal fragment. The broad substrate tolerance of PCY1 can be exploited as a biotechnological tool to generate structurally diverse arrays of macrocycles, including those with nonproteinogenic elements.
| + | |
| - | | + | |
| - | Characterization of the macrocyclase involved in the biosynthesis of RiPP cyclic peptides in plants.,Chekan JR, Estrada P, Covello PS, Nair SK Proc Natl Acad Sci U S A. 2017 Jun 20;114(25):6551-6556. doi:, 10.1073/pnas.1620499114. Epub 2017 Jun 5. PMID:28584123<ref>PMID:28584123</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5uw7" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Gypsophila vaccaria]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chekan, J R]] | + | [[Category: Chekan JR]] |
| - | [[Category: Nair, S K]] | + | [[Category: Nair SK]] |
| - | [[Category: Cyclase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Natural product]]
| + | |
| - | [[Category: Orbitide]]
| + | |
