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| | <StructureSection load='6v1v' size='340' side='right'caption='[[6v1v]], [[Resolution|resolution]] 3.19Å' scene=''> | | <StructureSection load='6v1v' size='340' side='right'caption='[[6v1v]], [[Resolution|resolution]] 3.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6v1v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V1V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v1v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V1V FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">vip3Bc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1428 "Bacillus cereus var. thuringiensis" Smith et al. 1952])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.189Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1v OCA], [http://pdbe.org/6v1v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v1v RCSB], [http://www.ebi.ac.uk/pdbsum/6v1v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1v ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1v OCA], [https://pdbe.org/6v1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v1v RCSB], [https://www.ebi.ac.uk/pdbsum/6v1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A290WPI2_BACTU A0A290WPI2_BACTU] |
| - | Vegetatively expressed insecticidal proteins (VIPs) produced by Bacillus thuringiensis fall into several classes of which the third, VIP3, is known for their activity against several key Lepidopteran pests of commercial broad acre crops and because their mode of action does not overlap with that of crystalline insecticidal proteins. The details of the VIP3 structure and mode of action have remained obscure for the quarter century that has passed since their discovery. In the present article, we report the first crystal structure of a full-length VIP3 protein. Crystallization of this target required multiple rounds of construct optimization and screening-over 200 individual sequences were expressed and tested. This protein adopts a novel global fold that combines domains with hitherto unreported topology and containing elements seemingly borrowed from carbohydrate-binding domains, lectins, or from other insecticidal proteins.
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| - | | + | |
| - | Crystal structure of a Vip3B family insecticidal protein reveals a new fold and a unique tetrameric assembly.,Zheng M, Evdokimov AG, Moshiri F, Lowder C, Haas J Protein Sci. 2019 Dec 16. doi: 10.1002/pro.3803. PMID:31840313<ref>PMID:31840313</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6v1v" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus cereus var. thuringiensis smith et al. 1952]] | + | [[Category: Bacillus thuringiensis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Evdokimov, A G]] | + | [[Category: Evdokimov AG]] |
| - | [[Category: Haas, J]] | + | [[Category: Haas J]] |
| - | [[Category: Lowder, C]] | + | [[Category: Lowder C]] |
| - | [[Category: Moshiri, F]] | + | [[Category: Moshiri F]] |
| - | [[Category: Zheng, M]] | + | [[Category: Zheng M]] |
| - | [[Category: Insecticidal protein bacillus thuringiensis pore forming]]
| + | |
| - | [[Category: Toxin]]
| + | |
