6v6s

<table><tr><td colspan='2'>[[6v6s]] is a 38 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V6S FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6s OCA], [http://pdbe.org/6v6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6s RCSB], [http://www.ebi.ac.uk/pdbsum/6v6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6s ProSAT]</span></td></tr>

== Disease ==

[[http://www.uniprot.org/uniprot/GCP6_HUMAN GCP6_HUMAN]] Autosomal recessive chorioretinopathy-microcephaly syndrome. The disease is caused by mutations affecting the gene represented in this entry.

[[http://www.uniprot.org/uniprot/TBG1_HUMAN TBG1_HUMAN]] Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. [[http://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP3_HUMAN GCP3_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP2_HUMAN GCP2_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP5_HUMAN GCP5_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP6_HUMAN GCP6_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.<ref>PMID:11694571</ref>

The gamma-tubulin ring complex (gamma-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human gamma-TuRC at approximately 3.8 A resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the gamma-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the gamma-TuRC lumen. Despite its asymmetric architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the gamma-TuRC subunits introduces large (&gt;100,000 A(2)) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the gamma-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.

 

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== References ==

[[Category: Chait, B T]]

[[Category: Kapoor, T M]]

[[Category: Molloy, K R]]

[[Category: Ti, S]]

[[Category: Urnavicius, L]]

[[Category: Wieczorek, M]]

[[Category: Tubulin]]