6vqr

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Current revision (14:39, 6 March 2024) (edit) (undo)
 
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<StructureSection load='6vqr' size='340' side='right'caption='[[6vqr]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
<StructureSection load='6vqr' size='340' side='right'caption='[[6vqr]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6vqr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VQR FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6vqr]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VQR FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=R7M:(2R)-2-hydroxy-7-methoxy-2-(pentafluoroethyl)-2,3-dihydro-4H-1-benzopyran-4-one'>R7M</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.78&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF3D7_0316600 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=R7M:(2R)-2-hydroxy-7-methoxy-2-(pentafluoroethyl)-2,3-dihydro-4H-1-benzopyran-4-one'>R7M</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqr OCA], [https://pdbe.org/6vqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vqr RCSB], [https://www.ebi.ac.uk/pdbsum/6vqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vqr OCA], [https://pdbe.org/6vqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vqr RCSB], [https://www.ebi.ac.uk/pdbsum/6vqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vqr ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/O77389_PLAF7 O77389_PLAF7]
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The intra-erythrocyte stage of P. falciparum relies primarily on glycolysis to generate adenosine triphosphate (ATP) and the energy required to support growth and reproduction. Lactic acid, a metabolic byproduct of glycolysis, is potentially toxic as it lowers the pH inside the parasite. Plasmodium falciparum formate-nitrite transporter (PfFNT), a 34-kDa transmembrane protein, has been identified as a novel drug target as it exports lactate from inside the parasite to the surrounding parasitophorous vacuole within the erythrocyte cytosol. The structure and detailed molecular mechanism of this membrane protein are not yet available. Here we present structures of PfFNT in the absence and presence of the functional inhibitor MMV007839 at resolutions of 2.56 A and 2.78 A using single-particle cryo-electron microscopy. Genetic analysis and transport assay indicate that PfFNT is able to transfer lactate across the membrane. Combined, our data suggest a stepwise displacement mechanism for substrate transport. The PfFNT membrane protein is capable of picking up lactate ions from the parasite's cytosol, converting them to lactic acids and then exporting these acids into the extracellular space.
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Structural basis of transport and inhibition of the Plasmodium falciparum transporter PfFNT.,Lyu M, Su CC, Kazura JW, Yu EW EMBO Rep. 2021 Jan 20:e51628. doi: 10.15252/embr.202051628. PMID:33471955<ref>PMID:33471955</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6vqr" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Plaf7]]
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Lyu, M]]
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[[Category: Lyu M]]
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[[Category: Su, C C]]
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[[Category: Su CC]]
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[[Category: Membrane protein]]
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[[Category: Transporter]]
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Current revision

CryoEM Structure of the PfFNT-inhibitor complex

PDB ID 6vqr

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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