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| | <StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]], [[Resolution|resolution]] 6.68Å' scene=''> | | <StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]], [[Resolution|resolution]] 6.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6vy0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Spitz Spitz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vy0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila_DSM_6578 Spirochaeta thermophila DSM 6578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vxz|6vxz]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.68Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Spith_0644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=869211 SPITZ])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [https://pdbe.org/6vy0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [https://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [http://pdbe.org/6vy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G0GA88_SPITZ G0GA88_SPITZ] |
| - | SthK, a cyclic nucleotide-modulated ion channel from Spirochaeta thermophila, activates slowly upon cAMP increase. This is reminiscent of the slow, cAMP-induced activation reported for the hyperpolarization-activated and cyclic nucleotide-gated channel HCN2 in the family of so-called pacemaker channels. Here, we investigate slow cAMP-induced activation in purified SthK channels using stopped-flow assays, mutagenesis, enzymatic catalysis and inhibition assays revealing that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK. We propose that SthK exists in two forms: trans Pro300 SthK with high ligand binding affinity and fast activation, and cis Pro300 SthK with low affinity and slow activation. Following channel activation, the cis/trans equilibrium, catalyzed by prolyl isomerases, is shifted towards trans, while steady-state channel activity is unaffected. Our results reveal prolyl isomerization as a regulatory mechanism for SthK, and potentially eukaryotic HCN channels. This mechanism could contribute to electrical rhythmicity in cells.
| + | |
| | | | |
| - | Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel.,Schmidpeter PAM, Rheinberger J, Nimigean CM Nat Commun. 2020 Dec 16;11(1):6401. doi: 10.1038/s41467-020-20104-4. PMID:33328472<ref>PMID:33328472</ref>
| + | ==See Also== |
| - | | + | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6vy0" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Spitz]] | + | [[Category: Spirochaeta thermophila DSM 6578]] |
| - | [[Category: Nimigean, C M]] | + | [[Category: Nimigean CM]] |
| - | [[Category: Rheinberger, J]] | + | [[Category: Rheinberger J]] |
| - | [[Category: Schmidpeter, P A.M]] | + | [[Category: Schmidpeter PAM]] |
| - | [[Category: Transport protein]]
| + | |
