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| | <StructureSection load='6w6m' size='340' side='right'caption='[[6w6m]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='6w6m' size='340' side='right'caption='[[6w6m]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6w6m]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W6M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W6M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6w6m]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W6M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w6m OCA], [http://pdbe.org/6w6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w6m RCSB], [http://www.ebi.ac.uk/pdbsum/6w6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w6m ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w6m OCA], [https://pdbe.org/6w6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w6m RCSB], [https://www.ebi.ac.uk/pdbsum/6w6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w6m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q9KNV0_VIBCH Q9KNV0_VIBCH] |
| - | Natural transformation is the process by which bacteria take up genetic material from their environment and integrate it into their genome by homologous recombination. It represents one mode of horizontal gene transfer and contributes to the spread of traits like antibiotic resistance. In Vibrio cholerae, a type IVa pilus (T4aP) is thought to facilitate natural transformation by extending from the cell surface, binding to exogenous DNA, and retracting to thread this DNA through the outer membrane secretin, PilQ. Here, we use a functional tagged allele of VcPilQ purified from native V. cholerae cells to determine the cryoEM structure of the VcPilQ secretin in amphipol to ~2.7 A. We use bioinformatics to examine the domain architecture and gene neighborhood of T4aP secretins in Proteobacteria in comparison with VcPilQ. This structure highlights differences in the architecture of the T4aP secretin from the type II and type III secretion system secretins. Based on our cryoEM structure, we design a series of mutants to reversibly regulate VcPilQ gate dynamics. These experiments support the idea of VcPilQ as a potential druggable target and provide insight into the channel that DNA likely traverses to promote the spread of antibiotic resistance via horizontal gene transfer by natural transformation.
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| - | | + | |
| - | CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae.,Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ Nat Commun. 2020 Oct 8;11(1):5080. doi: 10.1038/s41467-020-18866-y. PMID:33033258<ref>PMID:33033258</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6w6m" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Vibrio cholerae]] | | [[Category: Vibrio cholerae]] |
| - | [[Category: Sazinsky, M H]] | + | [[Category: Sazinsky MH]] |
| - | [[Category: Weaver, S J]] | + | [[Category: Weaver SJ]] |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Secretion system]]
| + | |
| - | [[Category: Transport protein]]
| + | |
