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| | <StructureSection load='6wa6' size='340' side='right'caption='[[6wa6]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6wa6' size='340' side='right'caption='[[6wa6]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6wa6]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_vr-2282 Atcc vr-2282]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WA6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WA6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wa6]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_pneumoniae Chlamydia pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WA6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lcrD, CP_0434, CPn_0323, BN1224_CV15_B_02600, BN1224_GiD_A_03360, BN1224_H12_DC_00060, BN1224_MUL2216_E_00600, BN1224_Panola_E_01450, BN1224_PB1_B_03220, BN1224_U1271_C_01630, BN1224_UZG1_A_03350, BN1224_Wien2_E_01080, BN1224_YK41_BG_00210, CWL029c_C_01630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83558 ATCC VR-2282])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wa6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wa6 OCA], [http://pdbe.org/6wa6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wa6 RCSB], [http://www.ebi.ac.uk/pdbsum/6wa6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wa6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wa6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wa6 OCA], [https://pdbe.org/6wa6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wa6 RCSB], [https://www.ebi.ac.uk/pdbsum/6wa6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wa6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q9Z8L5_CHLPN Q9Z8L5_CHLPN] |
| - | Type III protein secretion systems (T3SS) deliver effector proteins from the Gram-negative bacterial cytoplasm into a eukaryotic host cell through a syringe-like, multi-protein nanomachine. Cytosolic components of T3SS include a portion of the export apparatus, which traverses the inner membrane and features the opening of the secretion channel, and the sorting complex for substrate recognition and for providing the energetics required for protein secretion. Two components critical for efficient effector export are the export gate protein and the ATPase, which are proposed to be linked by the central stalk protein of the ATPase. We present the structure of the soluble export gate homo-nonamer, CdsV, in complex with the central stalk protein, CdsO, of its cognate ATPase, both derived from Chlamydia pneumoniae. This structure defines the interface between these essential T3S proteins and reveals that CdsO engages the periphery of the export gate that may allow the ATPase to catalyze an opening between export gate subunits to allow cargo to enter the export apparatus. We also demonstrate through structure-based mutagenesis of the homologous export gate in Pseudomonas aeruginosa that mutation of this interface disrupts effector secretion. These results provide novel insights into the molecular mechanisms governing active substrate recognition and translocation through a T3SS.
| + | |
| - | | + | |
| - | "The structure of the Type III secretion system export gate with CdsO, an ATPase lever arm".,Jensen JL, Yamini S, Rietsch A, Spiller BW PLoS Pathog. 2020 Oct 13;16(10):e1008923. doi: 10.1371/journal.ppat.1008923., eCollection 2020 Oct. PMID:33048983<ref>PMID:33048983</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6wa6" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc vr-2282]] | + | [[Category: Chlamydia pneumoniae]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jensen, J L]] | + | [[Category: Jensen JL]] |
| - | [[Category: Spiller, B W]] | + | [[Category: Spiller BW]] |
| - | [[Category: Effector secretion]]
| + | |
| - | [[Category: Protein secretion]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Type iii secretion]]
| + | |
