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| | <StructureSection load='6wcb' size='340' side='right'caption='[[6wcb]], [[Resolution|resolution]] 3.17Å' scene=''> | | <StructureSection load='6wcb' size='340' side='right'caption='[[6wcb]], [[Resolution|resolution]] 3.17Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6wcb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WCB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WCB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wcb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WCB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.17Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TMEM175 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wcb OCA], [http://pdbe.org/6wcb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wcb RCSB], [http://www.ebi.ac.uk/pdbsum/6wcb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wcb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wcb OCA], [https://pdbe.org/6wcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wcb RCSB], [https://www.ebi.ac.uk/pdbsum/6wcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wcb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/TM175_HUMAN TM175_HUMAN]] Disease susceptibility may be associated with variations affecting the gene represented in this entry. TMEM175 defects result in unstable lysosomal pH, leading to decreased lysosomal catalytic activity, decreased glucocerebrosidase activity, impaired autophagosome clearance by the lysosome and decreased mitochondrial respiration (PubMed:28193887).<ref>PMID:28193887</ref> | + | [https://www.uniprot.org/uniprot/TM175_HUMAN TM175_HUMAN] Disease susceptibility may be associated with variations affecting the gene represented in this entry. TMEM175 defects result in unstable lysosomal pH, leading to decreased lysosomal catalytic activity, decreased glucocerebrosidase activity, impaired autophagosome clearance by the lysosome and decreased mitochondrial respiration (PubMed:28193887).<ref>PMID:28193887</ref> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TM175_HUMAN TM175_HUMAN]] Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.<ref>PMID:26317472</ref> <ref>PMID:28723891</ref> | + | [https://www.uniprot.org/uniprot/TM175_HUMAN TM175_HUMAN] Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.<ref>PMID:26317472</ref> <ref>PMID:28723891</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Transmembrane protein 175 (TMEM175) is a K(+)-selective ion channel expressed in lysosomal membranes, where it establishes a membrane potential essential for lysosomal function and its dysregulation is associated with the development of Parkinson's Disease. TMEM175 is evolutionarily distinct from all known channels, predicting novel ion-selectivity and gating mechanisms. Here we present cryo-EM structures of human TMEM175 in open and closed conformations, enabled by resolutions up to 2.6 A. Human TMEM175 adopts a homodimeric architecture with a central ion-conduction pore lined by the side chains of the pore-lining helices. Conserved isoleucine residues in the center of the pore serve as the gate in the closed conformation. In the widened channel in the open conformation, these same residues establish a constriction essential for K(+) selectivity. These studies reveal the mechanisms of permeation, selectivity and gating and lay the groundwork for understanding the role of TMEM175 in lysosomal function.
| + | |
| - | | + | |
| - | Gating and selectivity mechanisms for the lysosomal K(+) channel TMEM175.,Oh S, Paknejad N, Hite RK Elife. 2020 Mar 31;9. pii: 53430. doi: 10.7554/eLife.53430. PMID:32228865<ref>PMID:32228865</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6wcb" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hite, R K]] | + | [[Category: Hite RK]] |
| - | [[Category: Oh, S]] | + | [[Category: Oh S]] |
| - | [[Category: Paknejad, N]] | + | [[Category: Paknejad N]] |
| - | [[Category: Ion channel]]
| + | |
| - | [[Category: Lysosome]]
| + | |
| - | [[Category: Potassium channel]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Disease
TM175_HUMAN Disease susceptibility may be associated with variations affecting the gene represented in this entry. TMEM175 defects result in unstable lysosomal pH, leading to decreased lysosomal catalytic activity, decreased glucocerebrosidase activity, impaired autophagosome clearance by the lysosome and decreased mitochondrial respiration (PubMed:28193887).[1]
Function
TM175_HUMAN Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[2] [3]
References
- ↑ Jinn S, Drolet RE, Cramer PE, Wong AH, Toolan DM, Gretzula CA, Voleti B, Vassileva G, Disa J, Tadin-Strapps M, Stone DJ. TMEM175 deficiency impairs lysosomal and mitochondrial function and increases alpha-synuclein aggregation. Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2389-2394. doi:, 10.1073/pnas.1616332114. Epub 2017 Feb 13. PMID:28193887 doi:http://dx.doi.org/10.1073/pnas.1616332114
- ↑ Cang C, Aranda K, Seo YJ, Gasnier B, Ren D. TMEM175 Is an Organelle K(+) Channel Regulating Lysosomal Function. Cell. 2015 Aug 27;162(5):1101-12. doi: 10.1016/j.cell.2015.08.002. PMID:26317472 doi:http://dx.doi.org/10.1016/j.cell.2015.08.002
- ↑ Lee C, Guo J, Zeng W, Kim S, She J, Cang C, Ren D, Jiang Y. The lysosomal potassium channel TMEM175 adopts a novel tetrameric architecture. Nature. 2017 Jul 27;547(7664):472-475. doi: 10.1038/nature23269. Epub 2017 Jul, 19. PMID:28723891 doi:http://dx.doi.org/10.1038/nature23269
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