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| | <StructureSection load='6wpg' size='340' side='right'caption='[[6wpg]], [[Resolution|resolution]] 2.28Å' scene=''> | | <StructureSection load='6wpg' size='340' side='right'caption='[[6wpg]], [[Resolution|resolution]] 2.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6wpg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WPG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wpg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.283Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NPR4, At4g19660, T16H5.20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpg OCA], [http://pdbe.org/6wpg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wpg RCSB], [http://www.ebi.ac.uk/pdbsum/6wpg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpg OCA], [https://pdbe.org/6wpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpg RCSB], [https://www.ebi.ac.uk/pdbsum/6wpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NPR4_ARATH NPR4_ARATH]] May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Involved in the regulation of basal defense responses against pathogens, and may be implicated in the cross-talk between the SA- and JA-dependent signaling pathways.<ref>PMID:15634206</ref> <ref>PMID:17076807</ref> | + | [https://www.uniprot.org/uniprot/NPR4_ARATH NPR4_ARATH] May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Involved in the regulation of basal defense responses against pathogens, and may be implicated in the cross-talk between the SA- and JA-dependent signaling pathways.<ref>PMID:15634206</ref> <ref>PMID:17076807</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
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| - | Salicylic acid (SA) is a plant hormone that is critical for resistance to pathogens(1-3). The NPR proteins have previously been identified as SA receptors(4-10), although how they perceive SA and coordinate hormonal signalling remain unknown. Here we report the mapping of the SA-binding core of Arabidopsis thaliana NPR4 and its ligand-bound crystal structure. The SA-binding core domain of NPR4 refolded with SA adopts an alpha-helical fold that completely buries SA in its hydrophobic core. The lack of a ligand-entry pathway suggests that SA binding involves a major conformational remodelling of the SA-binding core of NPR4, which we validated using hydrogen-deuterium-exchange mass spectrometry analysis of the full-length protein and through SA-induced disruption of interactions between NPR1 and NPR4. We show that, despite the two proteins sharing nearly identical hormone-binding residues, NPR1 displays minimal SA-binding activity compared to NPR4. We further identify two surface residues of the SA-binding core, the mutation of which can alter the SA-binding ability of NPR4 and its interaction with NPR1. We also demonstrate that expressing a variant of NPR4 that is hypersensitive to SA could enhance SA-mediated basal immunity without compromising effector-triggered immunity, because the ability of this variant to re-associate with NPR1 at high levels of SA remains intact. By revealing the structural mechanisms of SA perception by NPR proteins, our work paves the way for future investigation of the specific roles of these proteins in SA signalling and their potential for engineering plant immunity.
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| - | | + | |
| - | Structural basis of salicylic acid perception by Arabidopsis NPR proteins.,Wang W, Withers J, Li H, Zwack PJ, Rusnac DV, Shi H, Liu L, Yan S, Hinds TR, Guttman M, Dong X, Zheng N Nature. 2020 Aug 12. pii: 10.1038/s41586-020-2596-y. doi:, 10.1038/s41586-020-2596-y. PMID:32788727<ref>PMID:32788727</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 6wpg" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dong, X]] | + | [[Category: Dong X]] |
| - | [[Category: Guttman, M]] | + | [[Category: Guttman M]] |
| - | [[Category: Hinds, T R]] | + | [[Category: Hinds TR]] |
| - | [[Category: Li, H]] | + | [[Category: Li H]] |
| - | [[Category: Liu, L]] | + | [[Category: Liu L]] |
| - | [[Category: Rusnac, D V]] | + | [[Category: Rusnac DV]] |
| - | [[Category: Shi, H]] | + | [[Category: Shi H]] |
| - | [[Category: Wang, W]] | + | [[Category: Wang W]] |
| - | [[Category: Withers, J]] | + | [[Category: Withers J]] |
| - | [[Category: Yan, S]] | + | [[Category: Yan S]] |
| - | [[Category: Zheng, N]] | + | [[Category: Zheng N]] |
| - | [[Category: Zwack, P J]] | + | [[Category: Zwack PJ]] |
| - | [[Category: Arabidopsis]]
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| - | [[Category: Npr4]]
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| - | [[Category: Plant immunity]]
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| - | [[Category: Plant protein]]
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| - | [[Category: Receptor]]
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| - | [[Category: Sa]]
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| - | [[Category: Salicylic acid]]
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