6xqk

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Current revision (14:51, 6 March 2024) (edit) (undo)
 
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<StructureSection load='6xqk' size='340' side='right'caption='[[6xqk]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
<StructureSection load='6xqk' size='340' side='right'caption='[[6xqk]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6xqk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XQK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6xqk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XQK FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCY1, REG1, SRA1, YIL033C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xqk OCA], [https://pdbe.org/6xqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xqk RCSB], [https://www.ebi.ac.uk/pdbsum/6xqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xqk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xqk OCA], [https://pdbe.org/6xqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xqk RCSB], [https://www.ebi.ac.uk/pdbsum/6xqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xqk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST]] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
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[https://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein kinase A (PKA) is a widespread enzyme that plays a key role in many signaling pathways from lower eukaryotes to metazoans. In mammals, the regulatory (R) subunits sequester and target the catalytic (C) subunits to proper subcellular locations. This targeting is accomplished by the dimerization and docking (D/D) domain of the R subunits. The activation of the holoenzyme depends on the binding of the second messenger cAMP. The only available structures of the D/D domain proceed from mammalian sources. Unlike dimeric mammalian counterparts, the R subunit from Saccharomyces cerevisiae (Bcy1) forms tetramers in solution. Here we describe the first high-resolution structure of a non-mammalian D/D domain. The tetramer in the crystals of the Bcy1 D/D domain is a dimer of dimers that retain the classical D/D domain fold. By using phylogenetic and structural analyses combined with site-directed mutagenesis, we found that fungal R subunits present an insertion of a single amino acid at the D/D domain that shifts the position of a downstream, conserved arginine. This residue participates in intra-dimer interactions in mammalian D/D domains, while due to this insertion it is involved in inter-dimer contacts in Bcy1, which are crucial for the stability of the tetramer. This surprising finding challenges well-established concepts regarding the oligomeric state within the PKAR protein family and provides important insights into the yet unexplored structural diversity of the D/D domains and the molecular determinants of R subunit oligomerization.
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The crystal structure of yeast regulatory subunit reveals key evolutionary insights into Protein Kinase A oligomerization.,Bardeci NG, Tofolon E, Trajtenberg F, Caramelo J, Larrieux N, Rossi S, Buschiazzo A, Moreno S J Struct Biol. 2021 Apr 2:107732. doi: 10.1016/j.jsb.2021.107732. PMID:33819633<ref>PMID:33819633</ref>
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==See Also==
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*[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6xqk" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 18824]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Bardeci, N Gonzalez]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Buschiazzo, A]]
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[[Category: Buschiazzo A]]
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[[Category: Larrieux, N]]
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[[Category: Gonzalez Bardeci N]]
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[[Category: Trajtenberg, F]]
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[[Category: Larrieux N]]
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[[Category: Protein kinase some]]
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[[Category: Trajtenberg F]]
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[[Category: Signaling protein]]
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Current revision

Crystal structure of the D/D domain of PKA from S. cerevisiae

PDB ID 6xqk

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