This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1qu1
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1qu1.gif|left|200px]] | [[Image:1qu1.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1qu1", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | | | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | + | --> | |
| - | + | {{STRUCTURE_1qu1| PDB=1qu1 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE OF EHA2 (23-185)''' | '''CRYSTAL STRUCTURE OF EHA2 (23-185)''' | ||
| Line 29: | Line 26: | ||
[[Category: Skehel, J J.]] | [[Category: Skehel, J J.]] | ||
[[Category: Wiley, D C.]] | [[Category: Wiley, D C.]] | ||
| - | [[Category: | + | [[Category: Hemagglutinin]] |
| - | [[Category: | + | [[Category: Low-ph]] |
| - | [[Category: | + | [[Category: Virus/viral protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:42:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:42, 3 May 2008
CRYSTAL STRUCTURE OF EHA2 (23-185)
Overview
The structure of a stable recombinant ectodomain of influenza hemagglutinin HA(2) subunit, EHA(2) (23-185), defined by proteolysis studies of the intact bacterial-expressed ectodomain, was determined to 1.9-A resolution by using x-ray crystallography. The structure reveals a domain composed of N- and C-terminal residues that form an N cap terminating both the N-terminal alpha-helix and the central coiled coil. The N cap is formed by a conserved sequence, and part of it is found in the neutral pH conformation of HA. The C-terminal 23 residues of the ectodomain form a 72-A long nonhelical structure ordered to within 7 residues of the transmembrane anchor. The structure implies that continuous alpha helices are not required for membrane fusion at either the N or C termini. The difference in stability between recombinant molecules with and without the N cap sequences suggests that additional free energy for membrane fusion may become available after the formation of the central triple-stranded coiled coil and insertion of the fusion peptide into the target membrane.
About this Structure
1QU1 is a Single protein structure of sequence from Influenza a virus. The following page contains interesting information on the relation of 1QU1 with [Hemagglutinin]. Full crystallographic information is available from OCA.
Reference
N- and C-terminal residues combine in the fusion-pH influenza hemagglutinin HA(2) subunit to form an N cap that terminates the triple-stranded coiled coil., Chen J, Skehel JJ, Wiley DC, Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8967-72. PMID:10430879 Page seeded by OCA on Sat May 3 06:42:20 2008
