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5t7e

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<StructureSection load='5t7e' size='340' side='right'caption='[[5t7e]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='5t7e' size='340' side='right'caption='[[5t7e]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5t7e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_hygroscopicus"_jensen_1931 "actinomyces hygroscopicus" jensen 1931]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T7E FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5t7e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_hygroscopicus Streptomyces hygroscopicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T7E FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PPQ:PHOSPHINOTHRICIN'>PPQ</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[5t7d|5t7d]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PPQ:PHOSPHINOTHRICIN'>PPQ</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bar ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1912 "Actinomyces hygroscopicus" Jensen 1931])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transferase Transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.183 2.3.1.183] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7e OCA], [https://pdbe.org/5t7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t7e RCSB], [https://www.ebi.ac.uk/pdbsum/5t7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7e ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7e OCA], [https://pdbe.org/5t7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t7e RCSB], [https://www.ebi.ac.uk/pdbsum/5t7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7e ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/PAT_STRHY PAT_STRHY]] Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.<ref>PMID:16453790</ref>
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[https://www.uniprot.org/uniprot/PAT_STRHY PAT_STRHY] Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.<ref>PMID:16453790</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops (1-4) . Although early in vitro enzyme assays showed that recombinant BAR and PAT exhibit substrate preference toward phosphinothricin over the 20 proteinogenic amino acids (1) , indirect effects of BAR-containing transgenes in planta, including modified amino acid levels, have been seen but without the identification of their direct causes (5,6) . Combining metabolomics, plant genetics and biochemical approaches, we show that transgenic BAR indeed converts two plant endogenous amino acids, aminoadipate and tryptophan, to their respective N-acetylated products in several plant species. We report the crystal structures of BAR, and further delineate structural basis for its substrate selectivity and catalytic mechanism. Through structure-guided protein engineering, we generated several BAR variants that display significantly reduced non-specific activities compared with its wild-type counterpart in vivo. The transgenic expression of enzymes can result in unintended off-target metabolism arising from enzyme promiscuity. Understanding such phenomena at the mechanistic level can facilitate the design of maximally insulated systems featuring heterologously expressed enzymes.
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Non-specific activities of the major herbicide-resistance gene BAR.,Christ B, Hochstrasser R, Guyer L, Francisco R, Aubry S, Hortensteiner S, Weng JK Nat Plants. 2017 Dec;3(12):937-945. doi: 10.1038/s41477-017-0061-1. Epub 2017 Nov, 27. PMID:29180815<ref>PMID:29180815</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5t7e" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Actinomyces hygroscopicus jensen 1931]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Transferase]]
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[[Category: Streptomyces hygroscopicus]]
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[[Category: Christ, B]]
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[[Category: Christ B]]
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[[Category: Weng, J K]]
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[[Category: Weng JK]]
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[[Category: Phosphinothricin-deactivating activity gcn5-related n-acetyltransferase]]
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Revision as of 15:37, 6 March 2024

Crystal structure of Streptomyces hygroscopicus Bialaphos Resistance (BAR) protein in complex with Coenzyme A and L-phosphinothricin

PDB ID 5t7e

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