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| | <SX load='5tcq' size='340' side='right' viewer='molstar' caption='[[5tcq]], [[Resolution|resolution]] 3.60Å' scene=''> | | <SX load='5tcq' size='340' side='right' viewer='molstar' caption='[[5tcq]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tcq]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TCQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tcq]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TCQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tcp|5tcp]], [[5tcr|5tcr]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcq OCA], [http://pdbe.org/5tcq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tcq RCSB], [http://www.ebi.ac.uk/pdbsum/5tcq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tcq OCA], [https://pdbe.org/5tcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tcq RCSB], [https://www.ebi.ac.uk/pdbsum/5tcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tcq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/INVG_SALTY INVG_SALTY]] Involved in the invasion of the cells of the intestinal epithelium. Could be necessary for the export of invasion related determinants. | + | [https://www.uniprot.org/uniprot/SCTC1_SALTY SCTC1_SALTY] Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:9786184, PubMed:27974800, PubMed:30242280). Forms a ring-shaped multimeric structure with an apparent central pore in the outer membrane (PubMed:9786184, PubMed:21385715, PubMed:27974800, PubMed:30242280).<ref>PMID:21385715</ref> <ref>PMID:27974800</ref> <ref>PMID:30242280</ref> <ref>PMID:9786184</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes and that of the infected host cell. The internal channel formed by the injectisome allows for the direct delivery of partially unfolded virulence effectors into the host cytoplasm. The structural foundation of the injectisome is the basal body, a molecular lock-nut structure composed predominantly of three proteins that form highly oligomerized concentric rings spanning the inner and outer membranes. Here we present the structure of the prototypical Salmonella enterica serovar Typhimurium pathogenicity island 1 basal body, determined using single-particle cryo-electron microscopy, with the inner-membrane-ring and outer-membrane-ring oligomers defined at 4.3 A and 3.6 A resolution, respectively. This work presents the first, to our knowledge, high-resolution structural characterization of the major components of the basal body in the assembled state, including that of the widespread class of outer-membrane portals known as secretins.
| + | |
| - | | + | |
| - | Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body.,Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC Nature. 2016 Dec 14. doi: 10.1038/nature20576. PMID:27974800<ref>PMID:27974800</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| - | <div class="pdbe-citations 5tcq" style="background-color:#fffaf0;"></div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Salmonella enterica subsp. enterica serovar typhimurium]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Bergeron, J R.C]] | + | [[Category: Bergeron JRC]] |
| - | [[Category: Hong, C]] | + | [[Category: Hong C]] |
| - | [[Category: Huang, R K]] | + | [[Category: Huang RK]] |
| - | [[Category: Strynadka, N C.J]] | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Vuckovic, M]] | + | [[Category: Vuckovic M]] |
| - | [[Category: Worrall, L J]] | + | [[Category: Worrall LJ]] |
| - | [[Category: Yu, Z]] | + | [[Category: Yu Z]] |
| - | [[Category: Bacterial]]
| + | |
| - | [[Category: Injectisome]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Secretion]]
| + | |
| Structural highlights
Function
SCTC1_SALTY Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:9786184, PubMed:27974800, PubMed:30242280). Forms a ring-shaped multimeric structure with an apparent central pore in the outer membrane (PubMed:9786184, PubMed:21385715, PubMed:27974800, PubMed:30242280).[1] [2] [3] [4]
References
- ↑ Schraidt O, Marlovits TC. Three-dimensional model of Salmonella's needle complex at subnanometer resolution. Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715 doi:10.1126/science.1199358
- ↑ Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC. Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. Nature. 2016 Dec 14. doi: 10.1038/nature20576. PMID:27974800 doi:http://dx.doi.org/10.1038/nature20576
- ↑ Hu J, Worrall LJ, Hong C, Vuckovic M, Atkinson CE, Caveney N, Yu Z, Strynadka NCJ. Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin. Nat Commun. 2018 Sep 21;9(1):3840. doi: 10.1038/s41467-018-06298-8. PMID:30242280 doi:http://dx.doi.org/10.1038/s41467-018-06298-8
- ↑ Crago AM, Koronakis V. Salmonella InvG forms a ring-like multimer that requires the InvH lipoprotein for outer membrane localization. Mol Microbiol. 1998 Oct;30(1):47-56. doi: 10.1046/j.1365-2958.1998.01036.x. PMID:9786184 doi:http://dx.doi.org/10.1046/j.1365-2958.1998.01036.x
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