1quw
From Proteopedia
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'''SOLUTION STRUCTURE OF THE THIOREDOXIN FROM BACILLUS ACIDOCALDARIUS''' | '''SOLUTION STRUCTURE OF THE THIOREDOXIN FROM BACILLUS ACIDOCALDARIUS''' | ||
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[[Category: Rossi, M.]] | [[Category: Rossi, M.]] | ||
[[Category: Tato, M.]] | [[Category: Tato, M.]] | ||
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| - | [[Category: | + | [[Category: Thiol-disulfide]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:44:02 2008'' | |
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Revision as of 03:44, 3 May 2008
SOLUTION STRUCTURE OF THE THIOREDOXIN FROM BACILLUS ACIDOCALDARIUS
Overview
The thioredoxin (Trx) from Bacillus acidocaldarius (BacTrx), an eubacterium growing optimally at 333 K, is the first Trx described to date from a moderate thermophilic source. To understand the molecular basis of its thermostability, the three-dimensional structure in the oxidized form was determined by NMR methods. A total of 2276 1H-NMR derived distance constraints along with 23 hydrogen-bonds, 72 phi and 27 chi1 torsion angle restraints, were used in a protocol employing simulated annealing followed by restrained molecular dynamics and restrained energy minimization. BacTrx consists of a well-defined core region of five strands of beta-sheet, surrounded by four exposed alpha-helices, features shared by other members of the thioredoxin family. The BacTrx 3D structure was compared with the Escherichia coli Trx (EcTrx) determined by X-ray crystallographic diffraction, and a number of structural differences were observed that may contribute to its thermostabilty. The results of structural analysis indicated that protein stability is due to cumulative effects, the main factor being an increased number of ionic interactions cross-linking different secondary structural elements and clamping the C-terminal alpha-helix to the core of the protein.
About this Structure
1QUW is a Single protein structure of sequence from Alicyclobacillus acidocaldarius. Full crystallographic information is available from OCA.
Reference
NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius possible determinants of protein stability., Nicastro G, De Chiara C, Pedone E, Tato M, Rossi M, Bartolucci S, Eur J Biochem. 2000 Jan;267(2):403-13. PMID:10632710 Page seeded by OCA on Sat May 3 06:44:02 2008
