1qwj
From Proteopedia
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| - | + | {{STRUCTURE_1qwj| PDB=1qwj | SCENE= }} | |
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'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase''' | '''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase''' | ||
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[[Category: Muenster-Kuehnel, A K.]] | [[Category: Muenster-Kuehnel, A K.]] | ||
[[Category: Tiralongo, J.]] | [[Category: Tiralongo, J.]] | ||
| - | [[Category: | + | [[Category: Cmp-5-n-acetylneuraminic acid synthetase]] |
| - | [[Category: | + | [[Category: Cmp-neu5ac]] |
| - | [[Category: | + | [[Category: Glycosylation]] |
| - | [[Category: | + | [[Category: Lipopolysaccharide biosynthesis]] |
| - | [[Category: | + | [[Category: Sialic acid]] |
| - | [[Category: | + | [[Category: Sugar-activating enzyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:46:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:46, 3 May 2008
The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase
Overview
Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically active domain of the murine 5-N-acetylneuraminic acid synthetase in complex with the reaction product. In contrast to the previously solved structure of 5-N-acetylneuraminic acid synthetase from Neisseria meningitidis and the related CMP-KDO-synthetase of Escherichia coli, the murine enzyme is a tetramer, which was observed with the active sites closed. In this conformation a loop is shifted by 6A towards the active site and thus an essential arginine residue can participate in catalysis. Furthermore, a network of intermolecular salt-bridges and hydrogen bonds in the dimer as well as hydrophobic interfaces between two dimers indicate a cooperative behaviour of the enzyme. In addition, a complex regulation of the enzyme activity is proposed that includes phosphorylation and dephosphorylation.
About this Structure
1QWJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:14636592 Page seeded by OCA on Sat May 3 06:46:57 2008
Categories: Mus musculus | N-acylneuraminate cytidylyltransferase | Single protein | Gerardy-Schahn, R. | Huber, R. | Jacob, U. | Kaiser, J T. | Krapp, S. | Muenster-Kuehnel, A K. | Tiralongo, J. | Cmp-5-n-acetylneuraminic acid synthetase | Cmp-neu5ac | Glycosylation | Lipopolysaccharide biosynthesis | Sialic acid | Sugar-activating enzyme
