1x2i

<table><tr><td colspan='2'>[[1x2i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43587 Atcc 43587]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X2I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1X2I FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j22|1j22]], [[1j23|1j23]], [[1j24|1j24]], [[1j25|1j25]], [[1wp9|1wp9]]</div></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF2015 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 ATCC 43587])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1x2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x2i OCA], [http://pdbe.org/1x2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x2i RCSB], [http://www.ebi.ac.uk/pdbsum/1x2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x2i ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

XPF/Rad1/Mus81/Hef proteins recognize and cleave branched DNA structures. XPF and Rad1 proteins cleave the 5' side of nucleotide excision repair bubble, while Mus81 and Hef cleave similar sites of the nicked Holliday junction, fork, or flap structure. These proteins all function as dimers and consist of catalytic and helix-hairpin-helix DNA binding (HhH) domains. We have determined the crystal structure of the HhH domain of Pyrococcus furiosus Hef nuclease (HefHhH), which revealed the distinct mode of protein dimerization. Our structural and biochemical analyses also showed that each of the catalytic and HhH domains binds to distinct regions within the fork-structured DNA: each HhH domain from two separate subunits asymmetrically binds to the arm region, while the catalytic domain binds near the junction center. Upon binding to DNA, Hef nuclease disrupts base pairs near the cleavage site. It is most likely that this bipartite binding mode is conserved in the XPF/Rad1/Mus81 nuclease family.

Structural and functional analyses of an archaeal XPF/Rad1/Mus81 nuclease: asymmetric DNA binding and cleavage mechanisms.,Nishino T, Komori K, Ishino Y, Morikawa K Structure. 2005 Aug;13(8):1183-92. PMID:16084390<ref>PMID:16084390</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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== References ==

<references/>

[[Category: Atcc 43587]]

[[Category: Ishino, Y]]

[[Category: Komori, K]]

[[Category: Morikawa, K]]

[[Category: Nishino, T]]

[[Category: Hydrolase]]