1qwn

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[[Image:1qwn.gif|left|200px]]
[[Image:1qwn.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1qwn |SIZE=350|CAPTION= <scene name='initialview01'>1qwn</scene>, resolution 1.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1qwn", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GUL:5-FLUORO-BETA-L-GULOSYL+FLUORIDE'>GUL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_1,3-1,6-alpha-mannosidase Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.114 3.2.1.114] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ALPHA-MAN-II OR GMII OR CG18474/CG18802/CG8139 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])
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-->
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|DOMAIN=
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{{STRUCTURE_1qwn| PDB=1qwn | SCENE= }}
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|RELATEDENTRY=[[1hty|1HTY]], [[1hww|1HWW]], [[1hxk|1HXK]], [[1ps3|1PS3]], [[1qx1|1QX1]], [[1qwu|1QWU]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwn OCA], [http://www.ebi.ac.uk/pdbsum/1qwn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qwn RCSB]</span>
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}}
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'''GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride'''
'''GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride'''
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[[Category: Withers, S G.]]
[[Category: Withers, S G.]]
[[Category: 2 c-terminal beta barrel]]
[[Category: 2 c-terminal beta barrel]]
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[[Category: family 38 glycosyl hydrolase]]
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[[Category: Family 38 glycosyl hydrolase]]
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[[Category: n-terminal alpha-beta domain]]
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[[Category: N-terminal alpha-beta domain]]
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[[Category: three helix bundle]]
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[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:47:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:20:01 2008''
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Revision as of 03:47, 3 May 2008

Image:1qwn.gif

Template:STRUCTURE 1qwn

GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride


Overview

The family 38 golgi alpha-mannosidase II, thought to cleave mannosidic bonds through a double displacement mechanism involving a reaction intermediate, is a clinically important enzyme involved in glycoprotein processing. The structure of three different covalent glycosyl-enzyme intermediates have been determined to 1.2-A resolution for the Golgi alpha-mannosidase II from Drosophila melanogaster by use of fluorinated sugar analogues, both with the wild-type enzyme and a mutant enzyme in which the acid/base catalyst has been removed. All these structures reveal sugar intermediates bound in a distorted 1S5 skew boat conformation. The similarity of this conformation with that of the substrate in the recently determined structure of the Michaelis complex of a beta-mannanase (Ducros, V. M. A., Zechel, D. L., Murshudov, G. N., Gilbert, H. J., Szabo, L., Stoll, D., Withers, S. G., and Davies, G. J. (2002) Angew. Chem. Int. Ed. Engl. 41, 2824-2827) suggests that these disparate enzymes have recruited common stereoelectronic features in evolving their catalytic mechanisms.

About this Structure

1QWN is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates., Numao S, Kuntz DA, Withers SG, Rose DR, J Biol Chem. 2003 Nov 28;278(48):48074-83. Epub 2003 Sep 5. PMID:12960159 Page seeded by OCA on Sat May 3 06:47:19 2008

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OCA

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