2b2c
From Proteopedia
(Difference between revisions)
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<StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='2b2c' size='340' side='right'caption='[[2b2c]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2b2c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2C FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2c OCA], [https://pdbe.org/2b2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2c RCSB], [https://www.ebi.ac.uk/pdbsum/2b2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G5EFG3_CAEEL G5EFG3_CAEEL] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2c ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS. | ||
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| - | Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase.,Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:16226262<ref>PMID:16226262</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2b2c" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]] | *[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Caenorhabditis elegans]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Al-Karadaghi S]] | |
| - | [[Category: Al-Karadaghi | + | [[Category: Dufe VT]] |
| - | [[Category: Dufe | + | [[Category: Eschbach ML]] |
| - | [[Category: Eschbach | + | [[Category: Haider N]] |
| - | [[Category: Haider | + | [[Category: Karlberg T]] |
| - | [[Category: Karlberg | + | [[Category: Luersen K]] |
| - | [[Category: Luersen | + | [[Category: Walter RD]] |
| - | [[Category: Walter | + | |
| - | + | ||
| - | + | ||
Current revision
Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase
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